Product nameAnti-Peroxiredoxin 4 antibody
See all Peroxiredoxin 4 primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 4
Specificityab59542 reacts with Peroxiredoxin 4.
Tested applicationsSuitable for: WB, ELISA, IHC-Fr, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish
- Recombinant human Peroxiredoxin 4 protein (ab117192) can be used as a positive control in WB. Non-ciliated bronchiolar cells (Clara cells) in rat lung and sebaceous glands in rat skin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab59542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/4000. Predicted molecular weight: 31 kDa.|
|ELISA||1/1000 - 1/4000.|
|IHC-Fr||1/500 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
|IP||Use at an assay dependent concentration.|
FunctionProbably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Sequence similaritiesBelongs to the ahpC/TSA family.
Contains 1 thioredoxin domain.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
All lanes : Anti-Peroxiredoxin 4 antibody (ab59542) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 74 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The band observed at 27 kDa could potentially be a cleaved form of Peroxiredoxin 4 due to the presence of a 39 amino acid signal peptide.
Peroxiredoxin 4 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µl of Rabbit polyclonal to Peroxiredoxin 4 sera and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59542.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 27kDa; Peroxiredoxin 4, non specific - as present in control (lane 2); 27kDa: We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest.
ab59542 at 1/100 dilution staining Peroxiredoxin 4 in sebaceous glands in rat skin by Immunohistochemistry. Secondary antibody Jackson biotinylated Donkey anti-rabbit (1/2000) followed by Vector ABC kit and visualised with DAB substrate.
ab59542 at 1/100 dilution staining Peroxiredoxin 4 in Non-ciliated bronchiolar cells (Clara cells) in rat lung by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
This product has been referenced in:
- Zhang H et al. Differential expression of peroxiredoxin 3 in laryngeal squamous cell carcinoma. Oncotarget 8:3471-3480 (2017). WB . Read more (PubMed: 27966448) »
- Jayaram DT et al. Nanoparticle-induced oxidation of corona proteins initiates an oxidative stress response in cells. Nanoscale 9:7595-7601 (2017). Read more (PubMed: 28537609) »