Key features and details
- Rabbit polyclonal to Peroxiredoxin 5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Peroxiredoxin 5 antibody
See all Peroxiredoxin 5 primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human Peroxiredoxin 5 aa 164-214. The exact sequence is proprietary.
Database link: P30044
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226468 was affinity purified using an epitope specific to Peroxiredoxin 5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionReduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peroxiredoxin 2 family.
Contains 1 thioredoxin domain.
Cellular localizationMitochondrion. Cytoplasm. Peroxisome.
- Information by UniProt
- ACR1 antibody
- Alu co repressor 1 antibody
- Alu corepressor 1 antibody
All lanes : Anti-Peroxiredoxin 5 antibody (ab226468) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Peroxiredoxin 5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226468 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226468 at 1 µg/ml.
Lane 1: ab226468 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 75 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226468 has not yet been referenced specifically in any publications.