Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Overview

  • Product name

    Anti-Peroxiredoxin 5 antibody [EPR14528(B)]
    See all Peroxiredoxin 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 5 aa 50-150. The exact sequence is proprietary.
    Database link: P30044

  • Positive control

    • HepG2, HeLa, Jurkat and A549 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/60.

Target

  • Function

    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

Images

  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180123 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

  • Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

References

ab180123 has not yet been referenced specifically in any publications.

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