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Cell Biology Apoptosis Intracellular Associated Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
  • Immunocytochemistry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
  • Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human

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Protein
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Recombinant human Peroxiredoxin 5 protein (ab168029)

View more associated products

Overview

  • Product name

    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free
    See all Peroxiredoxin 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250136 is the carrier-free version of ab180123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14528(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Associated products

  • Alternative Versions

    • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human Peroxiredoxin 5 protein (ab168029)
  • Related Products

    • Recombinant human Peroxiredoxin 5 protein (ab168029)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IP
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Mitochondrion. Cytoplasm. Peroxisome.
  • Target information above from: UniProt accession P30044 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 25824 Human
    • Entrez Gene: 54683 Mouse
    • Entrez Gene: 113898 Rat
    • Omim: 606583 Human
    • SwissProt: P30044 Human
    • SwissProt: P99029 Mouse
    • SwissProt: Q9R063 Rat
    • Unigene: 502823 Human
    • Unigene: 279782 Mouse
    • Unigene: 2944 Rat
    see all
  • Alternative names

    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

Images

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

    This data was developed using ab180123, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab180123, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Immunocytochemistry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
  • Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab250136? Please let us know so that we can cite the reference in this datasheet.

ab250136 has not yet been referenced specifically in any publications.

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