Recombinant Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free
- Suitable for: ICC, WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human
Overview
-
Product name
Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free
See all Peroxiredoxin 5 primary antibodies -
Description
Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250136 is the carrier-free version of ab180123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14528(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
|
Application | Abreviews | Notes |
---|---|---|
ICC |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
---|
ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa). |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peroxiredoxin 2 family.
Contains 1 thioredoxin domain. -
Cellular localization
Mitochondrion. Cytoplasm. Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 25824 Human
- Entrez Gene: 54683 Mouse
- Entrez Gene: 113898 Rat
- Omim: 606583 Human
- SwissProt: P30044 Human
- SwissProt: P99029 Mouse
- SwissProt: Q9R063 Rat
- Unigene: 502823 Human
see all -
Alternative names
- ACR1 antibody
- Alu co repressor 1 antibody
- Alu corepressor 1 antibody
see all
Images
-
This data was developed using ab180123, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: A549 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab180123, the same antibody clone in a different buffer formulation.
-
This data was developed using ab180123, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).
-
This data was developed using ab180123, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab250136 has not yet been referenced specifically in any publications.