Overview

  • Product name
    Anti-Peroxiredoxin 6 antibody
    See all Peroxiredoxin 6 primary antibodies
  • Description
    Rabbit polyclonal to Peroxiredoxin 6
  • Host species
    Rabbit
  • Specificity
    This antibody is specific to the sulfinic and sulfonic form of PrxVI.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Sulfonylated peptide (KLH coupled) corresponding to the active site sequence common to mammalian Peroxiredoxin 6.

  • Positive control
    • HeLa cell lysate.

Applications

Our Abpromise guarantee covers the use of ab28444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 25 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 18826942

Target

  • Function
    Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
  • Sequence similarities
    Belongs to the ahpC/TSA family. Rehydrin subfamily.
    Contains 1 thioredoxin domain.
  • Cellular localization
    Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 Cys antibody
    • 1 Cys peroxiredoxin antibody
    • 1 Cys PRX antibody
    • 1 cysPrx antibody
    • 1-Cys peroxiredoxin antibody
    • 1-Cys PRX antibody
    • 24 kDa protein antibody
    • 9430088D19Rik antibody
    • AA690119 antibody
    • Acidic calcium independent phospholipase A2 antibody
    • Acidic calcium-independent phospholipase A2 antibody
    • aiPLA2 antibody
    • Antioxidant protein 2 antibody
    • AOP2 antibody
    • Aop2 rs3 antibody
    • Brp 12 antibody
    • Ciliary body glutathione peroxidase antibody
    • CP 3 antibody
    • EC 1.11.1.15 antibody
    • EC 1.11.1.7 antibody
    • EC 3.1.1. antibody
    • Epididymis secretory sperm binding protein Li 128m antibody
    • GPx antibody
    • HEL S 128m antibody
    • KIAA0106 antibody
    • Liver 2D page spot 40 antibody
    • Ltw4 antibody
    • Lvtw 4 antibody
    • MGC46173 antibody
    • mKIAA0106 antibody
    • Non selenium glutathione peroxidase antibody
    • Non-selenium glutathione peroxidase antibody
    • NSGPx antibody
    • ORF06 antibody
    • OTTHUMP00000032693 antibody
    • p29 antibody
    • Peroxiredoxin-6 antibody
    • Peroxiredoxin6 antibody
    • PHGPx antibody
    • Phospholipase A2 lysosomal antibody
    • PLA2 antibody
    • PRDX 6 antibody
    • Prdx5 antibody
    • PRDX6 antibody
    • Prdx6 rs3 antibody
    • PRDX6_HUMAN antibody
    • PRX antibody
    • Red blood cells page spot 12 antibody
    • Thiol specific antioxidant protein antibody
    see all

Images

  • All lanes : Anti-Peroxiredoxin 6 antibody (ab28444) at 1/2000 dilution

    Lane 1 : H2O2 untreated HeLa cell lysates
    Lane 2 : H2O2 treated HeLa cell lysates
    Lane 3 : Reduced recombinant Peroxiredoxin 6
    Lane 4 : Oxidised recombinant Peroxiredoxin 6

    Predicted band size: 25 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti Peroxiredoxin 6 antibody

    Lane 1 : H2O2 untreated HeLa cell lysate
    Lane 2 : H2O2 treated HeLa cell lysate
    Lane 3 : Reduced recombinant Peroxiredoxin 6
    Lane 4 : Oxidised recombinant Peroxiredoxin 6

    Predicted band size: 25 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:
  • O'Flaherty C & de Souza AR Hydrogen peroxide modifies human sperm peroxiredoxins in a dose-dependent manner. Biol Reprod 84:238-47 (2011). WB ; Human . Read more (PubMed: 20864641) »
  • Kim SY  et al. H2O2-dependent hyperoxidation of peroxiredoxin 6 (Prdx6) plays a role in cellular toxicity via up-regulation of iPLA2 activity. J Biol Chem 283:33563-8 (2008). ICC/IF ; Human . Read more (PubMed: 18826942) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

rll yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you are also able toprovide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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