Product nameAnti-Peroxiredoxin 6 antibody
See all Peroxiredoxin 6 primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 6
Specificityab59543 reacts with Peroxiredoxin 6.
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein (Rat) Peroxiredoxin 6
- Rat and mouse lung and human brain.
General notesStaining pattern: cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. Stains human brain astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab59543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/4000. Predicted molecular weight: 25 kDa.|
|ELISA||1/1000 - 1/4000.|
|IHC-Fr||1/500 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain.
Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
- Information by UniProt
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
Lane 1 : Anti-Peroxiredoxin 6 antibody (ab59543)
Lane 2 : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/1000 dilution
All lanes : Mouse skeletal muscle whole tissue lysates
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Additional bands at: 25 kDa, 38, 45 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
ab59543 staining Peroxiredoxin 6 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 60 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.
ab59543 at 1/100 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by Immunohistochemistry. Secondary antibody anti-rabbit IgG conjugated to Cy3 (1/100).
ab59543 at 1/1000 dilution staining Peroxiredoxin 6 in astrocytes in Parkinson's disease by IHC-P. Secondary antibody Donkey anti-rabbit conjugated to biotin.
All lanes : Anti-Peroxiredoxin 6 antibody (ab59543) at 1/2500 dilution
Lane 1 : Wild-type rat cortex
Lanes 2-5 : Transgenic (APP/PS1) male mouse cortical lysate
Lanes 6-9 : Wild-type male mouse cortical lysate
All lanes : Alexa Fluor 680-conjugated goat anti rabbit IgG polyclonal antibody
Predicted band size: 25 kDa
Observed band size: ~25 kDa why is the actual band size different from the predicted?
Western blot showing detection of Peroxiredoxin 6 in mouse and rat cortical lysates by ab59543.
ICC/IF image of ab59543 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59543, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab59543 staining Peroxiredoxin 6 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
This product has been referenced in:
- Paul S et al. STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox Biol 14:423-438 (2018). WB ; Rat . Read more (PubMed: 29078168) »
- Rodger EJ et al. Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response. Biomed Res Int 2018:4963942 (2018). Read more (PubMed: 30596094) »