Product nameAnti-Peroxiredoxin 6 antibody
See all Peroxiredoxin 6 primary antibodies
DescriptionRabbit polyclonal to Peroxiredoxin 6
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Non human primates
Synthetic peptide corresponding to Human Peroxiredoxin 6 aa 150 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue, Lung (Rat) Tissue, HeLa Nuclear , Hela Whole Cell - Bleomycin Treated (40U/ml), Thymus (Rat) Tissue, Kidney (Mouse) Tissue, HepG2 Whole Cell
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionInvolved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Sequence similaritiesBelongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain.
Cellular localizationCytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles.
- Information by UniProt
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
All lanes : Anti-Peroxiredoxin 6 antibody (ab73683) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 30 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ICC/IF image of ab73683 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 1µg/ml.
IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73683, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
ab73683 has not yet been referenced specifically in any publications.