Overview

  • Product name
    Anti-Peroxiredoxin-SO3 antibody
  • Description
    Rabbit polyclonal to Peroxiredoxin-SO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic sulfonylated peptide (Human) conjugated to KLH, corresponding to Prx I-IV active site.

  • Positive control
    • H2O2 treated HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 22 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18826942

Target

  • Relevance
    Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin 3 antibody
    • Peroxiredoxin 4 antibody
    • PRDX1 antibody
    • PRDX2 antibody
    • PRDX3 antibody
    • PRDX4 antibody
    see all

Images

  • Upper blot - Prx-SO3 antibody (ab16830) at 1/2000
    Lower blot - Prx1 antibody as a loading control

    Lane 1: H2O2 untreated HeLa cell lysate
    Lane 2: H2O2 treated HeLa cell lysate
    Lane 3: Reduced recombinant Prx1
    Lane 4: Oxidized recombinant Prx1

  • IHC image of ab16830 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16830,1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Carroll B  et al. Oxidation of SQSTM1/p62 mediates the link between redox state and protein homeostasis. Nat Commun 9:256 (2018). Read more (PubMed: 29343728) »
  • Putker M  et al. Mammalian Circadian Period, But Not Phase and Amplitude, Is Robust Against Redox and Metabolic Perturbations. Antioxid Redox Signal 28:507-520 (2018). Read more (PubMed: 28506121) »
See all 43 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Aorta)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Specification
Aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. D Richard

Verified customer

Submitted Jul 27 2015

Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (TCA prep)
Gel Running Conditions
Reduced Denaturing (4 -12% NuPAGE bis-tris gel with MES buffer and antioxidant)
Loading amount
10 µg
Treatment
With or without 1mM H2O2 for 15 minutes
Specification
TCA prep
Blocking step
0.5% BSA and 0.5% milk in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C

Dr. Helen Causton

Verified customer

Submitted Apr 22 2015

Application
IHC - Wholemount
Sample
Rat Tissue (Mesenteric Venule)
Specification
Mesenteric Venule

Abcam user community

Verified customer

Submitted Dec 18 2012

Answer

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies; therefore, it is important to verify the storage conditions for each of our products when you receive them.

Our AbPromise guarantee applies if you are using the product in species and applications listed on the datasheet and contact us within six months of purchase and the antibody is not performing as indicated on the datasheet. In these scenarios, we are happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:
https://www.abcam.com/index.html?pageconfig=abpromise

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for contacting Abcam and I am sorry for the late response.
I have talked to the lab about the protocol that was used to oxidize the PrX1 protein (ab16830) and they recommended fallowing the protocol in the attached reference.
Please let me know if there is anything else I can help you with.

Read More

Answer

Thank you for contacting us with your questions. We have only tested these antibodies under reducing conditions in WB. They may be suitable for use under non-reducing conditions as well, but this has not been validated in-house. I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Answer

Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary antibody ab97141 will react with the primary antibody ab62391 and is suitable for use in WB.  You can detect this secondary antibody by either conjugating it with one of our EasyLink conjugating kits, or using an additional anti-goat secondary antibody.  Alternatively, you could use the equivalent HRP conjugated anti-Chicken IgY secondary antibody ab97150,  I hope this helps, please let me know if you need any additional information. 

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount
30 µg
Specification
Mouse Embryonic Fibroblasts
Treatment
100 uM H2O2 for 6 hrs
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary neurons)
Loading amount
600000 cells
Specification
primary neurons
Treatment
H2O2 100uM 1h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Dec 21 2006

Question
Answer

Thank you for your enquiry. I can confirm that milk was used as a blocking agent to test ab16830 by western blotting. Please let me know if you have any further inquiries.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up