Application
Western blot
Sample
Rat Cell lysate - whole cell (primary neurons)
Loading amount
600000 cells
Specification
primary neurons
Treatment
H2O2 100uM 1h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 22, 24, 26 kDa Non-specific: 19, 32 36, 42, 45, 4 kDa
Positive control
Cells treated with H2O2 100 uM 1h
Negative control
Untreated cells
Additional data
Abcam response
Thank you for your feedback.
Please note that the recommended dilution for this antibody is 1/2000, and you are using it at 1/1000. This may account for the non-specific bands you are seeing on the blot. You may wish to try the antibody at this dilution to see if this improves your results.
Please note that the recommended dilution for this antibody is 1/2000, and you are using it at 1/1000. This may account for the non-specific bands you are seeing on the blot. You may wish to try the antibody at this dilution to see if this improves your results.
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Verified customer
Submitted Dec 21 2006