Peroxynitrite Assay Kit (Cell-based, Flow cytometry) (ab233470)

Overview

  • Product name

    Peroxynitrite Assay Kit (Cell-based, Flow cytometry)
    See all Peroxynitrite kits
  • Detection method

    Fluorescent
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite (ONOO-) in biological systems. In order to address this unmet need, ab233470 Peroxynitrite Assay Kit (Cell-based, Flow cytometry) provides a sensitive tool to monitor ONOO- levels in living cells. Peroxynitrite Sensor Green is developed as an excellent fluorescent probe, which can specifically react with intercellular ONOO- to generate a bright green fluorescent product. This kit is optimized for flow cytometry.

  • Notes

    Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a broad range of physiological and pathological processes.

  • Platform

    Microplate reader, Flow cytometer

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    DMSO 1 x 100µl
    Peroxynitrite Sensor Green 2 vials

Images

  • (A) Jurkat cells were co-incubated with Peroxynitrite Sensor Green and 200 µM SIN-1 in full medium at 37 ºC for 1 hour.

    (B) Cells were pre-stained with Peroxynitrite Sensor Green for 1 hour, washed with PBS and then incubated with 200 µM SIN-1 in full medium at 37 ºC for 16 hours.

    Cells stained with Peroxynitrite Sensor Green but without SIN-1 treatment were used as a control. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer in the FITC channel.

Protocols

References

ab233470 has not yet been referenced specifically in any publications.

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