Product namePeroxynitrite Assay Kit (Fluorometric)
See all Peroxynitrite kits
Assay typeCell-based (quantitative)
Peroxynitrite Assay Kit (Fluorometric) (ab233469) provides a sensitive tool to measure peroxynitrite in solution.
Peroxynitrite Sensor Green reacts with ONOO¯ to generate a bright green fluorescent product. It specifically reacts with ONOO¯ with high selectivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS). This kit can be used with a fluorescence microplate reader and spectrometer.
Peroxynitrite (ONOO¯) is a strong oxidizing species and a highly active nitrating agent. It is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and quantify peroxynitrite in solution.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Assay Buffer 1 x 20ml DMSO 1 x 100µl Peroxynitrite Green reagent 1 vial
Peroxynitrite was measured with the Peroxynitrite Quantification Kit (Fluorometric) (ab233469) on a solid black 96-well plate using a Gemini microplate reader. As low as 1.25 μM was detected. (Δ Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point.)
ab233469 has not yet been referenced specifically in any publications.