Overview

  • Product name

    Peroxynitrite Assay Kit (Fluorometric)
    See all Peroxynitrite kits
  • Detection method

    Fluorescent
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Peroxynitrite Assay Kit (Fluorometric) (ab233469) provides a sensitive tool to measure peroxynitrite in solution.


    Peroxynitrite Sensor Green reacts with ONOO¯ to generate a bright green fluorescent product. It specifically reacts with ONOO¯ with high selectivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS). This kit can be used with a fluorescence microplate reader and spectrometer.

  • Notes

    Peroxynitrite (ONOO¯) is a strong oxidizing species and a highly active nitrating agent. It is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and quantify peroxynitrite in solution.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Buffer 1 x 20ml
    DMSO 1 x 100µl
    Peroxynitrite Green reagent 1 vial

Images

  • Peroxynitrite was measured with the Peroxynitrite Quantification Kit (Fluorometric) (ab233469) on a solid black 96-well plate using a Gemini microplate reader. As low as 1.25 μM was detected. (Δ Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence intensity value of the blank wells for each data point.)

Protocols

References

ab233469 has not yet been referenced specifically in any publications.

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