Immunocytochemistry/ Immunofluorescence abreview for Anti-PERP antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mammary gland)
Specification
Mammary gland
Fixative
Acetone
Permeabilization
Yes - TritonX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/100
Incubation time
7 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Donkey Serum in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexafluor 488 Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
This antibody was also tried using 4% PFA fixation and cryo/paraffin embedding but did not work.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As this product had not yet been tested in WB on mouse we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.

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Verified customer

Submitted May 24 2012

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