Recombinant
Recombinant Anti-PERP antibody [EPR7885] (ab129083)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PERP antibody [EPR7885]
See all PERP primary antibodies -
Description
Rabbit monoclonal [EPR7885] to PERP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues at the C terminus of Human PERP (UniProt ID: Q96FX8).
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Positive control
- Mouse heart, Rat heart, A431, HACAT and Human skin lysates; A431 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7885 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 21 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. -
Tissue specificity
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts. -
Sequence similarities
Belongs to the TMEM47 family. -
Cellular localization
Cell junction > desmosome. Cell membrane. Associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 64065 Human
- Entrez Gene: 64058 Mouse
- Entrez Gene: 292949 Rat
- Omim: 609301 Human
- SwissProt: Q96FX8 Human
- SwissProt: Q9JK95 Mouse
- SwissProt: Q561R0 Rat
- Unigene: 201446 Human
see all -
Alternative names
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
see all
Images
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![Western blot - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143040-anti-perp-antibody-epr7885-western-blot.jpg)
Western blot - Anti-PERP antibody [EPR7885] (ab129083)All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : A431 lysate
Lane 4 : HACAT lysate
Lane 5 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143039-anti-perp-antibody-epr7885-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)ab129083, at a dilution of 1/100, staining PERP in A431 cells by Immunofluorescence. -
![Other - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143038-anti-perp-antibody-epr7885.jpg)
Other - Anti-PERP antibody [EPR7885] (ab129083)
Datasheets and documents
References
ab129083 has not yet been referenced specifically in any publications.
