Recombinant Anti-PERP antibody [EPR7885] (ab129083)
![Western blot - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-395378-anti-perp-antibody-epr7885-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PERP antibody [EPR7885] (ab129083)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7885] to PERP
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PERP antibody [EPR7885]
See all PERP primary antibodies -
Description
Rabbit monoclonal [EPR7885] to PERP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, HaCaT and HeLa cell lysates; Mouse heart, rat heart and human skin lysates. IHC-P: A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7885 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. -
Tissue specificity
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts. -
Sequence similarities
Belongs to the TMEM47 family. -
Cellular localization
Cell junction > desmosome. Cell membrane. Associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 64065 Human
- Entrez Gene: 64058 Mouse
- Entrez Gene: 292949 Rat
- Omim: 609301 Human
- SwissProt: Q96FX8 Human
- SwissProt: Q9JK95 Mouse
- SwissProt: Q561R0 Rat
- Unigene: 201446 Human
see all -
Alternative names
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
see all
Images
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Western blot - Anti-PERP antibody [EPR7885] (ab129083)All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PERP knockout HeLa cell lysate
Lane 3 : HaCaT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab129083 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129083 Anti-PERP antibody [EPR7885] was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265335 (knockout cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143039-anti-perp-antibody-epr7885-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (ab129083)ab129083, at a dilution of 1/100, staining PERP in A431 cells by Immunofluorescence. -
![Western blot - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143040-anti-perp-antibody-epr7885-western-blot.jpg)
Western blot - Anti-PERP antibody [EPR7885] (ab129083)All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat heart lysate
Lane 3 : A431 lysate
Lane 4 : HACAT lysate
Lane 5 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa -
![OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-143038-anti-perp-antibody-epr7885.jpg)
OI-RD Scanning - Anti-PERP antibody [EPR7885] (ab129083) -
![Anti-PERP antibody [EPR7885] (ab129083)](https://www.abcam.com/ps/products/129/ab129083/Images/ab129083-5-benefits-of-recombinant-antibodies.png)
Anti-PERP antibody [EPR7885] (ab129083)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab129083 has been referenced in 1 publication.
- Zhang Y et al. Comparative transcriptome analysis reveals PERP upregulated during Salmonella Enteritidis challenge in laying ducks. J Cell Physiol 234:11330-11347 (2019). PubMed: 30478915
