Recombinant Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)
![Western blot - Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)](https://www.abcam.com/ps/products/248/ab248288/Images/ab248288-395379-anti-perp-antibody-epr7885-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7885] to PERP - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PERP antibody [EPR7885] - BSA and Azide free
See all PERP primary antibodies -
Description
Rabbit monoclonal [EPR7885] to PERP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HaCaT cell lysates.
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General notes
Ab248288 is the carrier-free version of ab129083. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248288 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 4.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7885 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 21 kDa. |
Target
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Function
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. -
Tissue specificity
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts. -
Sequence similarities
Belongs to the TMEM47 family. -
Cellular localization
Cell junction > desmosome. Cell membrane. Associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 64065 Human
- Entrez Gene: 64058 Mouse
- Entrez Gene: 292949 Rat
- Omim: 609301 Human
- SwissProt: Q96FX8 Human
- SwissProt: Q9JK95 Mouse
- SwissProt: Q561R0 Rat
- Unigene: 201446 Human
see all -
Alternative names
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
see all
Images
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Western blot - Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PERP knockout HeLa cell lysate
Lane 3 : HaCaT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab129083).
Lanes 1-3: Merged signal (red and green). Green - ab129083 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129083 Anti-PERP antibody [EPR7885] was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265335 (knockout cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)](https://www.abcam.com/ps/products/248/ab248288/Images/ab248288-2-benefits-of-recombinant-antibodies.png)
Anti-PERP antibody [EPR7885] - BSA and Azide free (ab248288)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab248288 has not yet been referenced specifically in any publications.