Anti-Pertussis toxin antibody (ab193494)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Pertussis toxin antibody -
Description
Rabbit polyclonal to Pertussis toxin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Bordetella pertussis -
Immunogen
Recombinant full length protein corresponding to Bordetella pertussis Pertussis toxin aa 35-269.
Sequence:DDPPATVYRYDSRPPEDVFQNGFTAWGNNDNVLDHLTGRSCQVGSSNSAF VSTSSSRRYTEVYLEHRMQEAVEAERAGRGTGHFIGYIYEVRADNNFYGA ASSYFEYVDTYGDNAGRILAGALATYQSEYLAHRRIPPENIRRVTRVYHN GITGETTTTEYSNARYVSQQTRANPNPYTSRRSVASIVGTLVRMAPVIGA CMARQAESSEAMAAWSERAGEAMVLVYYESIAYSF
Database link: P04977
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.03% Proclin
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab193494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 30 kDa. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordetella pertussis. PT catalyzes the ADP-ribosylation of the alpha subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction by preventing the G proteins from interacting with G protein-coupled receptors on the cell membrane. PT consists of one molecule each of S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecules of S4 (12 kDa). -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2665068 Bordetella pertussis
- Entrez Gene: 2665069 Bordetella pertussis
- Entrez Gene: 2665406 Bordetella pertussis
- Entrez Gene: 2665407 Bordetella pertussis
- Entrez Gene: 2665408 Bordetella pertussis
- SwissProt: P04977 Bordetella pertussis
- SwissProt: P04978 Bordetella pertussis
- SwissProt: P04979 Bordetella pertussis
see all -
Alternative names
- IAP S1 antibody
- IAP S2 antibody
- IAP S3 antibody
see all
Images
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Western blot - Anti-Pertussis toxin antibody (ab193494)Anti-Pertussis toxin antibody (ab193494) at 2 µg/ml + Recombinant Bordella pertussis pertussis toxin subunit 1 at 0.1 µg
Secondary
Polyclonal goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Protocols
References
ab193494 has not yet been referenced specifically in any publications.
