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    pertussis-toxin-antibody-ab193494.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Other gram-ve bacteria
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Anti-Pertussis toxin antibody (ab193494)

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Western blot - Anti-Pertussis toxin antibody (ab193494)

    Key features and details

    • Rabbit polyclonal to Pertussis toxin
    • Suitable for: WB, ELISA
    • Reacts with: Bordetella pertussis
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Pertussis toxin antibody
    • Description

      Rabbit polyclonal to Pertussis toxin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WB, ELISAmore details
    • Species reactivity

      Reacts with: Bordetella pertussis
    • Immunogen

      Recombinant full length protein corresponding to Bordetella pertussis Pertussis toxin aa 1 to the C-terminus.
      Database link: P04977

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Constituents: 49% PBS, 50% Glycerol, 0.03% Proclin 300
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      >95%
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Other gram-ve bacteria

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Related Products

      • Native B. pertussis Bordetella pertussis toxin/ptxD protein (ab124299)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab193494 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 30 kDa.
    ELISA
    Use at an assay dependent concentration.
    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 30 kDa.
    ELISA
    Use at an assay dependent concentration.

    Target

    • Relevance

      Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordetella pertussis. PT catalyzes the ADP-ribosylation of the alpha subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction by preventing the G proteins from interacting with G protein-coupled receptors on the cell membrane. PT consists of one molecule each of S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecules of S4 (12 kDa).
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 2665068 Bordetella pertussis
      • Entrez Gene: 2665069 Bordetella pertussis
      • Entrez Gene: 2665406 Bordetella pertussis
      • Entrez Gene: 2665407 Bordetella pertussis
      • Entrez Gene: 2665408 Bordetella pertussis
      • SwissProt: P04977 Bordetella pertussis
      • SwissProt: P04978 Bordetella pertussis
      • SwissProt: P04979 Bordetella pertussis
      • SwissProt: P04981 Bordetella pertussis
      • SwissProt: P0A3R5 Bordetella pertussis
      see all
    • Alternative names

      • IAP S1 antibody
      • IAP S2 antibody
      • IAP S3 antibody
      • IAP S4 antibody
      • IAP S5 antibody
      • Islet activating protein S1 antibody
      • Islet activating protein S2 antibody
      • Islet activating protein S3 antibody
      • Islet activating protein S4 antibody
      • Islet activating protein S5 antibody
      • NAD dependent ADP ribosyltransferase antibody
      • Pertussis toxin subunit 1 antibody
      • Pertussis toxin subunit 2 antibody
      • Pertussis toxin subunit 3 antibody
      • Pertussis toxin subunit 4 antibody
      • Pertussis toxin subunit 5 antibody
      • PTX S1 antibody
      • PTX S2 antibody
      • PTX S3 antibody
      • PTX S4 antibody
      • PTX S5 antibody
      • PtxA antibody
      • PtxB antibody
      • PtxC antibody
      • PtxD antibody
      • PtxE antibody
      see all

    Images

    • Western blot - Anti-Pertussis toxin antibody (ab193494)
      Western blot - Anti-Pertussis toxin antibody (ab193494)
      Anti-Pertussis toxin antibody (ab193494) at 2 µg/ml + Recombinant Bordella pertussis pertussis toxin subunit 1 at 0.1 µg

      Secondary
      Polyclonal goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 36 kDa why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab193494? Please let us know so that we can cite the reference in this datasheet.

    ab193494 has not yet been referenced specifically in any publications.

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