Product nameAnti-Pescadillo antibody
See all Pescadillo primary antibodies
DescriptionRabbit polyclonal to Pescadillo
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo (NP_055118.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Tissue specificitySignificant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
Sequence similaritiesBelongs to the pescadillo family.
Contains 1 BRCT domain.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
- Information by UniProt
- FLJ43790 antibody
- Homo sapiens pescadillo mRNA complete cds antibody
- PES antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pescadillo with ab72539 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-Pescadillo antibody (ab72539) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
This product has been referenced in:
- Qiu YB et al. PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERa/ERß protein ratio. Sci Rep 9:1032 (2019). Read more (PubMed: 30705367) »
- Rankin CR et al. Linear and circular CDKN2B-AS1 expression is associated with Inflammatory Bowel Disease and participates in intestinal barrier formation. Life Sci 231:116571 (2019). Read more (PubMed: 31207308) »