Product namePescadillo overexpression 293T lysate (whole cell)
See all Pescadillo lysates
ab94281 is a 293T cell transfected lysate in which Human Pescadillo has been transiently over-expressed using a pCMV-Pescadillo plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Tissue specificity: Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. Similarity: Belongs to the pescadillo family. Contains 1 BRCT domain. PTM: Sumoylated.
Our Abpromise guarantee covers the use of ab94281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94281 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Pescadillo antibody (ab88543) at 1/500 dilution
Lane 1 :
Pescadillo overexpression 293T lysate (whole cell) (ab94281)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94281 has not yet been referenced specifically in any publications.