PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
Key features and details
- PE/Texas Red® Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Texas Red®. Ex: 496nm, Em: 613nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE/Texas Red® Anti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies -
Description
PE/Texas Red® Rat monoclonal [53-6.7] to CD8 alpha -
Host species
Rat -
Conjugation
PE/Texas Red®. Ex: 496nm, Em: 613nm -
Specificity
ab25194 recognises CD8 alpha. In vivo and in vitro treatment with ab25294 effectively depletes CD8a+ cells. It also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD8 alpha. Spleen cells or thymocyte membranes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
53-6.7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.2µg for 106 cells.
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Notes |
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Flow Cyt
Use 0.2µg for 106 cells. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha with ab25294 at 0.1 µg/106cells and CD4 with ab25496.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab25294 has been referenced in 7 publications.
- Li W et al. Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy. Nat Commun 12:7264 (2021). PubMed: 34907171
- Yan J et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine 32:2833-42 (2014). PubMed: 24631084
- Tomov VT et al. Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses. J Virol 87:7015-31 (2013). PubMed: 23596300
- Hall JM et al. Chronic psychological stress suppresses contact hypersensitivity: Potential roles of dysregulated cell trafficking and decreased IFN-? production. Brain Behav Immun N/A:N/A (2013). Mouse . PubMed: 24184400
- Break TJ et al. Extracellular superoxide dismutase inhibits innate immune responses and clearance of an intracellular bacterial infection. J Immunol 188:3342-50 (2012). Flow Cyt ; Mouse . PubMed: 22393157
- Hall AO et al. The cytokines interleukin 27 and interferon-? promote distinct Treg cell populations required to limit infection-induced pathology. Immunity 37:511-23 (2012). PubMed: 22981537
- Meeks KD et al. IL-23 is required for protection against systemic infection with Listeria monocytogenes. J Immunol 183:8026-34 (2009). Flow Cyt ; Mouse . PubMed: 19923464