Key features and details
- PE/Texas Red® Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Texas Red®. Ex: 496nm, Em: 613nm
- Isotype: IgG2a
Product namePE/Texas Red® Anti-CD8 alpha antibody [53-6.7]
See all CD8 alpha primary antibodies
DescriptionPE/Texas Red® Rat monoclonal [53-6.7] to CD8 alpha
ConjugationPE/Texas Red®. Ex: 496nm, Em: 613nm
Specificityab25194 recognises CD8 alpha. In vivo and in vitro treatment with ab25294 effectively depletes CD8a+ cells. It also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Concentration information loading...
Light chain typekappa
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- Anti-CD8 alpha antibody [53-6.7] (ab25478)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
- PE/Cy5® Anti-CD8 alpha antibody [53-6.7] (ab95588)
Our Abpromise guarantee covers the use of ab25294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
Flow cytometry analysis of BALB/c mouse splenocytes labelling CD8 alpha with ab25294 at 0.1 µg/106cells and CD4 with ab25496.
ab25294 has been referenced in 6 publications.
- Yan J et al. Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine. Vaccine 32:2833-42 (2014). PubMed: 24631084
- Tomov VT et al. Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses. J Virol 87:7015-31 (2013). PubMed: 23596300
- Hall JM et al. Chronic psychological stress suppresses contact hypersensitivity: Potential roles of dysregulated cell trafficking and decreased IFN-? production. Brain Behav Immun N/A:N/A (2013). Mouse . PubMed: 24184400
- Break TJ et al. Extracellular superoxide dismutase inhibits innate immune responses and clearance of an intracellular bacterial infection. J Immunol 188:3342-50 (2012). Flow Cyt ; Mouse . PubMed: 22393157
- Hall AO et al. The cytokines interleukin 27 and interferon-? promote distinct Treg cell populations required to limit infection-induced pathology. Immunity 37:511-23 (2012). PubMed: 22981537
- Meeks KD et al. IL-23 is required for protection against systemic infection with Listeria monocytogenes. J Immunol 183:8026-34 (2009). Flow Cyt ; Mouse . PubMed: 19923464