Overview

  • Product name

  • Description

    Rabbit polyclonal to PEX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PEX1 aa 1233-1283. The exact sequence is proprietary.
    Sequence:

    HLMTALGH TRPSISEDDW KNFAELYESF QNPKRRKNQS GTMFRPGQKV TLA


    Database link: O43933

  • Positive control

    • HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH 3T3 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 142 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes.
  • Involvement in disease

    Peroxisome biogenesis disorder complementation group 1
    Peroxisome biogenesis disorder 1A
    Peroxisome biogenesis disorder 1B
    Heimler syndrome 1
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Cellular localization

    Cytoplasm. Peroxisome membrane. Associated with peroxisomal membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Highly similar to ATPase [H.sapiens] antibody
    • Peroxin 1 antibody
    • Peroxin-1 antibody
    • peroxin1 antibody
    • Peroxisome biogenesis disorder protein 1 antibody
    • Peroxisome biogenesis factor 1 antibody
    • PEX1 antibody
    • PEX1_HUMAN antibody
    • ZWS1 antibody
    see all

Images

  • Detection of PEX1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217059 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217059 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

  • All lanes : Anti-PEX1 antibody (ab217059) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : mouse TCMK1 whole cell lysate
    Lane 5 : mouse NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 142 kDa


    Exposure time: 3 minutes

References

ab217059 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab217059.
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