Key features and details
- Rabbit polyclonal to PEX1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PEX1 antibody
See all PEX1 primary antibodies
DescriptionRabbit polyclonal to PEX1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH 3T3 cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 142 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes.
Involvement in diseasePeroxisome biogenesis disorder complementation group 1
Peroxisome biogenesis disorder 1A
Peroxisome biogenesis disorder 1B
Heimler syndrome 1
Sequence similaritiesBelongs to the AAA ATPase family.
Cellular localizationCytoplasm. Peroxisome membrane. Associated with peroxisomal membranes.
- Information by UniProt
- Highly similar to ATPase [H.sapiens] antibody
- Peroxin 1 antibody
- Peroxin-1 antibody
Detection of PEX1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217059 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217059 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-PEX1 antibody (ab217059) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3 minutes
ab217059 has not yet been referenced specifically in any publications.