Overview

  • Product name
  • Description
    Rabbit polyclonal to PEX11A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 35-84 (KAGKEKVVMK LKKLESSVST GRKWFRLGNV VHAIQATEQS IHATDLVPRL) of Human PEX11A (NP_003838).

  • Positive control
    • OVCAR-3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab104959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in peroxisomal proliferation and may regulate peroxisomes division (PubMed:9792670). May mediate binding of coatomer proteins to the peroxisomal membrane (By similarity). Promotes membrane protrusion and elongation on the peroxisomal surface (PubMed:20826455).
  • Sequence similarities
    Belongs to the peroxin-11 family.
  • Post-translational
    modifications
    Seems not to be N-glycosylated.
  • Cellular localization
    Peroxisome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 28 kDa peroxisomal integral membrane protein antibody
    • HsPEX11p antibody
    • MGC119947 antibody
    • MGC138534 antibody
    • Peroxin-11A antibody
    • Peroxisomal biogenesis factor 11 alpha antibody
    • Peroxisomal biogenesis factor 11A antibody
    • Peroxisomal membrane protein 11A antibody
    • PEX11-alpha antibody
    • PEX11A antibody
    • PMP28 antibody
    • Protein PEX11 homolog alpha antibody
    • PX11A_HUMAN antibody
    see all

Images

  • Anti-PEX11A antibody (ab104959) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg

    Predicted band size: 28 kDa



    Gel concentration: 12%

References

This product has been referenced in:
  • Kanasaki M  et al. Deficiency in catechol-o-methyltransferase is linked to a disruption of glucose homeostasis in mice. Sci Rep 7:7927 (2017). Read more (PubMed: 28801594) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Human Liver)
Loading amount
20 µg
Specification
Human Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 20 2012

Question
Answer

DISCOUNT CODE: 100%ABR-EB3W1
Expiration date: 14/01/2013

I am very pleased to hear you would like to accept our offer and test ab104959 in mouse samples in western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our PEX11A antibodies

To our knowledge, these antibodies have not been tested in mouse samples in western blot. Therefore, I can offer a discount off a future purchase if you buy one of these products and test it in mouse samples and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

I have performed a BLAST search of the immunogens used to generate these 3 antibodies and ab104959 as the highest homology with the mouse protein at 87%. Therefore I would suggest testing this antibody. If you are interested in the testing discount offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab104959 in western blot. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase ab104959 either by phone, fax, or online (www.abcam.com).

3. Test it in western blot.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab104959 turns out to be unsuitable for western blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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