Anti-PEX14 antibody (ab183885)
Key features and details
- Rabbit polyclonal to PEX14
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PEX14 antibody
See all PEX14 primary antibodies -
Description
Rabbit polyclonal to PEX14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PEX14 aa 174-377. The exact sequence is proprietary.
Database link: O75381 -
Positive control
- WB: HEK-293T, A431, HeLa, Neuro-2a, C8-D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2 whole cell lysate; IF: Hela whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Component of the peroxisomal translocation machinery with PEX13 and PEX17. Interacts with both the PTS1 and PTS2 receptors. Binds directly to PEX17. -
Involvement in disease
Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) [MIM:601791]. PBD-CGK is a peroxisomal disorder arising from a failure of protein import into the peroxisomal membrane or matrix. The peroxisome biogenesis disorders (PBD group) are genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Include disorders are: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum (PBD-ZSS).
Defects in PEX14 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. -
Sequence similarities
Belongs to the peroxin-14 family. -
Cellular localization
Peroxisome membrane. - Information by UniProt
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Database links
- Entrez Gene: 5195 Human
- Entrez Gene: 56273 Mouse
- Entrez Gene: 64460 Rat
- Omim: 601791 Human
- SwissProt: O75381 Human
- SwissProt: Q9R0A0 Mouse
- SwissProt: Q642G4 Rat
- Unigene: 149983 Human
see all -
Alternative names
- dJ734G22.2 antibody
- NAPP2 antibody
- NF E2 associated polypeptide 2 antibody
see all
Images
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-PEX14 antibody (ab183885)Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells labeling PEX14 with ab183885 at 1/500 dilution (green) and Hoechst 33342 (blue). Cells were fixed in 4% paraformaldehyde at room temperature for 15 mins.
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS-PAGE
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Western blot - Anti-PEX14 antibody (ab183885)All lanes : Anti-PEX14 antibody (ab183885) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 41 kDa10% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab183885 has been referenced in 4 publications.
- Bentebbal SA et al. A human infertility-associated KASH5 variant promotes mitochondrial localization. Sci Rep 11:10133 (2021). PubMed: 33980926
- Federspiel JD et al. Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA. Cell Rep 32:107943 (2020). PubMed: 32726614
- Gu Y et al. Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. EMBO J 38:e101994 (2019). PubMed: 31625181
- Jean Beltran PM et al. Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication. Cell Host Microbe 24:526-541.e7 (2018). PubMed: 30269970