Anti-PEX19 antibody [EPR9266(B)] (ab137072)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-PEX19 antibody [EPR9266(B)]
See all PEX19 primary antibodies -
DescriptionRabbit monoclonal [EPR9266(B)] to PEX19
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, IP, ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide within Human PEX19 aa 1-100. The exact sequence is proprietary.
Database link: P40855 -
Positive control
- Jurkat, rat brain lysate, rat heart and MOLT4 cell lysates; Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR9266(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab137072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/10 - 1/100. | |
| ICC/IF | 1/50 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
Target
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FunctionNecessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
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Tissue specificityUbiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.
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Involvement in diseaseDefects in PEX19 are the cause of peroxisome biogenesis disorder complementation group 14 (PBD-CG14) [MIM:600279]; also known as PBD-CGJ. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies.
Defects in PEX19 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. -
Sequence similaritiesBelongs to the peroxin-19 family.
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Cellular localizationCytoplasm. Peroxisome membrane. Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes.
- Information by UniProt
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Database links
- Entrez Gene: 5824 Human
- Entrez Gene: 289233 Rat
- Omim: 600279 Human
- SwissProt: P40855 Human
- SwissProt: Q9QYU1 Rat
- Unigene: 517232 Human
- Unigene: 203104 Rat
- Unigene: 225447 Rat
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Alternative names
- 33 kDa housekeeping protein antibody
- D1S2223E antibody
- HK33 antibody
see all
Images
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![Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-4-ab137072WB2.jpg)
Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 33 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
The doublets are reported by PMID: 9339377, 28817674 and 10051604 -
![Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-5-ab137072ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PEX19 antibody [EPR9266(B)] (ab137072)Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-6-ab137072FC1.jpg)
Flow Cytometry - Anti-PEX19 antibody [EPR9266(B)] (ab137072)Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-3-ab137072WB1.jpg)
Western blot - Anti-PEX19 antibody [EPR9266(B)] (ab137072)All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : Rat brain lysates with 5% NFDM/TBST
Lane 3 : Rat heart lysates with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
The doublets are reported by PMID: 9339377, 28817674 and 10051604 -
![Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)](https://www.abcam.com/ps/products/137/ab137072/Images/ab137072-7-ab137072IP1.jpg)
Immunoprecipitation - Anti-PEX19 antibody [EPR9266(B)] (ab137072)ab137072 (purified) at 1:50 dilution (2µg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab137072 & MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Paudel I et al. Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines. Biochem J 475:3471-3492 (2018). Read more (PubMed: 30322886) »
- Wolfson RL et al. KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1. Nature 543:438-442 (2017). Read more (PubMed: 28199306) »
