Overview

  • Product name
    Anti-PEX19 antibody [EPR9266(B)]
    See all PEX19 primary antibodies
  • Description
    Rabbit monoclonal [EPR9266(B)] to PEX19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PEX19 aa 1-100. The exact sequence is proprietary.
    Database link: P40855

  • Positive control
    • Jurkat, rat brain lysate, rat heart and MOLT4 cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.
    • Tissue specificity
      Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.
    • Involvement in disease
      Defects in PEX19 are the cause of peroxisome biogenesis disorder complementation group 14 (PBD-CG14) [MIM:600279]; also known as PBD-CGJ. PBD refers to a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix. The PBD group is comprised of four disorders: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum. The PBD group is genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies.
      Defects in PEX19 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
    • Sequence similarities
      Belongs to the peroxin-19 family.
    • Cellular localization
      Cytoplasm. Peroxisome membrane. Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 33 kDa housekeeping protein antibody
      • D1S2223E antibody
      • HK33 antibody
      • Housekeeping gene 33kD antibody
      • OK/SW-cl.22 antibody
      • PBD12A antibody
      • Peroxin 19 antibody
      • Peroxin-19 antibody
      • Peroxisomal biogenesis factor 19 antibody
      • Peroxisomal farnesylated protein antibody
      • PEX19 antibody
      • PEX19_HUMAN antibody
      • PMP1 antibody
      • PMPI antibody
      • PXF antibody
      • PXMP1 antibody
      see all

    Images

    • All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/1000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
      Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 33 kDa
      Observed band size: 35,40 kDa
      why is the actual band size different from the predicted?



      The doublets are reported by PMID: 9339377, 28817674 and 10051604
    • Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling PEX19 with Purified ab137072 at 1:100 (10.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PEX19 with purified ab137072 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • All lanes : Anti-PEX19 antibody [EPR9266(B)] (ab137072) at 1/2000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : Rat brain lysates with 5% NFDM/TBST
      Lane 3 : Rat heart lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 35,40 kDa why is the actual band size different from the predicted?



      The doublets are reported by PMID: 9339377, 28817674 and 10051604
    • ab137072 (purified) at 1:50 dilution (2µg) immunoprecipitating PEX19 in MOLT-4 whole cell lysate.
      Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab137072 & MOLT-4 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137072 in MOLT-4 whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:
    • Paudel I  et al. Sab concentrations indicate chemotherapeutic susceptibility in ovarian cancer cell lines. Biochem J 475:3471-3492 (2018). Read more (PubMed: 30322886) »
    • Wolfson RL  et al. KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1. Nature 543:438-442 (2017). Read more (PubMed: 28199306) »
    See all 5 Publications for this product

    Customer reviews and Q&As

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Gel Running Conditions
    Reduced Denaturing (5% stacking and 10% separating Bis-Tris gel)
    Loading amount
    270000 cells
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Ms. Ani Akpinar

    Verified customer

    Submitted Feb 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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