Key features and details
- Rabbit polyclonal to PEX26
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PEX26 antibody
See all PEX26 primary antibodies
DescriptionRabbit polyclonal to PEX26
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: K562 whole cell lysate. IHC-P: Human colon cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
|WB||1/500 - 1/5000. Predicted molecular weight: 34 kDa.|
RelevancePEX26 is probably required for protein import into peroxisomes. It anchors PEX1 and PEX6 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes. Defects in the PEX26 gene are the cause of peroxisome biogenesis disorder complementation group 8 (PBD-CG8), which is a a group of peroxisomal disorders arising from a failure of protein import into the peroxisomal membrane or matrix.
Cellular localizationPeroxisome membrane.
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Anti-PEX26 antibody (ab235084) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Paraffin-embedded human colon cancer tissue stained for PEX26 using ab235084 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235084 has not yet been referenced specifically in any publications.