Anti-Pf1 antibody (ab219863)

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Overview

  • Product name

    Anti-Pf1 antibody
    See all Pf1 primary antibodies

  • Description

    Rabbit polyclonal to Pf1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human Pf1 aa 239-332.
    Sequence:

    GSSKRRRKEETTGKNVKKTQHELDHNGLVPLPVKVCFTCNRSCRVAPLIQ CDYCPLLFHMDCLEPPLTAMPLGRWMCPNHIEHVVLNQKNMTLS


    Database link: Q96QT6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • A549 cells.

  • General notes

    Previously labelled as PHF12. 

Properties

Applications

Our Abpromise guarantee covers the use of ab219863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Use PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Relevance

    Pf1 acts as a transcriptional repressor. It is involved in recruitment of functional SIN3A complexes to DNA. Pf1 represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). It may also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • FLJ34122 antibody
      • KIAA1523 antibody
      • MGC131914 antibody
      • Pf1 antibody
      • PHD factor 1 antibody
      • PHD finger protein 12 antibody
      • PHD zinc finger transcription factor antibody
      • PHF 12 antibody
      • PHF12 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Pf1 antibody (ab219863)
      Immunocytochemistry/ Immunofluorescence - Anti-Pf1 antibody (ab219863)

      Immunofluorescent analysis of A549 cells (PFA-fixed/Triton X-100 permeabilized) labeling Pf1 with ab219863 at 4 µg/ml (green).

    References

    ab219863 has not yet been referenced specifically in any publications.

    Publishing research using ab219863? Please let us know so that we can cite the reference in this datasheet.

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