Anti-PF4 antibody (ab9561)

Reviews (1)Q&A (1)References (6)

Overview

  • Product name
    Anti-PF4 antibody
    See all PF4 primary antibodies
  • Description
    Rabbit polyclonal to PF4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)

  • Positive control
    • Recombinant human PF4 protein (ab9562) can be used as a positive control in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab9561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required.
IHC-P 1/100.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to PF4 (Biotin) (ab83123).

Can be paired for Sandwich ELISA with ab83123

Target

  • Function
    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C-X-C motif chemokine 4 antibody
      • Chemokine (C X C motif) ligand 4 antibody
      • Chemokine (CXC motif) ligand 4 antibody
      • CXCL 4 antibody
      • CXCL4 antibody
      • Iroplact antibody
      • MGC138298 antibody
      • Oncostatin A antibody
      • Oncostatin-A antibody
      • OncostatinA antibody
      • PF 4 antibody
      • PF-4 antibody
      • PF4 antibody
      • Platelet factor 4 antibody
      • PLF4_HUMAN antibody
      • SCYB 4 antibody
      • SCYB4 antibody
      • short form antibody
      • Small inducible cytokine subfamily member 4 antibody
      see all

    Images

    • Western blot - Anti-PF4 antibody (ab9561)
      Western blot - Anti-PF4 antibody (ab9561)
      Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 11 kDa
      Observed band size: 11 kDa
      Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes

    References

    This product has been referenced in:
    • Maybin JA  et al. Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair. J Clin Endocrinol Metab 102:1851-1860 (2017). Read more (PubMed: 28323919) »
    • Izumi M  et al. Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty. Arthritis Res Ther 18:191 (2016). Human . Read more (PubMed: 27558507) »
    See all 6 Publications for this product

    Publishing research using ab9561? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I had a few question regarding platelet factor 4 antibody catalog number: ab9561. I need a neutralizing antibody that I can just add to my solution containing PF4. On you website it says that this antibody is neutralizing. I was wondering if this is tested and how much I need to use to neutralize PF4 in solution. Furthermore, I was wondering how much this product costs and if I can get any special quotes for ordering through University of British Columbia. I was also wondering if your company sells functional platelet factor 4 protein and how much that is.

    Read More
    Answer

    Thank you for contacting us.

    Ab9561 has been used in neutralizing assays and has been published byLambert MPet al.Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.Blood110:1153-60 (2007).PMID:17495129

    To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required. The exact conditions needed for you however will need to be determined experimentally.

    We currently offer this product at 100ul for $424.00 and do not have special arrangement with the University of British Columbia. You may order directly by clicking the "Add To Basket" button on the web page, by calling our customer service specialists at 1-888-772-2226 or by fax at 1-866-739-9884.

    We also offer an active hPF4, ab80477 (https://www.abcam.com/PF4-protein-Active-ab80477.html). This product is available in a 100ug size for $424.00.

    These products are covered by our Abpromise guarantee. If you experience any troubles using these products in the manner described on our datasheet and the product has been purchased within the last six months, we offer scientific support, replacement or refund under our Abpromise guarantee. More information about our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=10372

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Western blot abreview for Anti-PF4 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Platelet lysate)
    Loading amount
    50 µg
    Specification
    Platelet lysate
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris Hcl gel)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

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    Verified customer

    Submitted Aug 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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