Anti-PF4 antibody (ab9561)
Key features and details
- Rabbit polyclonal to PF4
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PF4 antibody
See all PF4 primary antibodies -
Description
Rabbit polyclonal to PF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PF4. Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)
Database link: P02776 -
Positive control
- Recombinant human PF4 protein (ab9562) can be used as a positive control in WB.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab49735
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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| Sandwich ELISA |
Use at an assay dependent concentration.
Can be paired for Sandwich ELISA with ab83123 |
| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with ab83123 |
Target
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Function
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsBinds non-covalently to a proteoglycan molecule. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5196 Human
- Omim: 173460 Human
- SwissProt: P02776 Human
- Unigene: 81564 Human
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Alternative names
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
Images
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Western blot - Anti-PF4 antibody (ab9561)Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Sandwich ELISA - Anti-PF4 antibody (ab9561)To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.
Datasheets and documents
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Datasheet download
References (10)
ab9561 has been referenced in 10 publications.
- Chanpeng P et al. Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in ß-thalassmia/HbE patients. Sci Rep 9:6059 (2019). PubMed: 30988349
- Lande R et al. CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-a production in systemic sclerosis. Nat Commun 10:1731 (2019). PubMed: 31043596
- Carestia A et al. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice. Cell Rep 28:896-908.e5 (2019). PubMed: 31340152
- Gollomp K et al. Neutrophil accumulation and NET release contribute to thrombosis in HIT. JCI Insight 3:N/A (2018). PubMed: 30232279
- Maybin JA et al. Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair. J Clin Endocrinol Metab 102:1851-1860 (2017). PubMed: 28323919
- Izumi M et al. Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty. Arthritis Res Ther 18:191 (2016). Human . PubMed: 27558507
- Du Y et al. Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiation. Mol Med Rep 14:2473-82 (2016). WB . PubMed: 27484039
- Fritzer A et al. Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domains. Biochem J 422:533-42 (2009). WB . PubMed: 19552626
- Houard X et al. Mediators of neutrophil recruitment in human abdominal aortic aneurysms. Cardiovasc Res 82:532-41 (2009). IHC-P ; Human . PubMed: 19201759
- Lambert MP et al. Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications. Blood 110:1153-60 (2007). Neutralising ; Human . PubMed: 17495129
