Overview

  • Product name
    Anti-PF4 antibody
    See all PF4 primary antibodies
  • Description
    Rabbit polyclonal to PF4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)

Properties

Applications

Our Abpromise guarantee covers the use of ab9561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required.
IHC-P 1/100.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to PF4 (Biotin) (ab83123).

Can be paired for Sandwich ELISA with ab83123

Target

  • Function
    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • C-X-C motif chemokine 4 antibody
      • Chemokine (C X C motif) ligand 4 antibody
      • Chemokine (CXC motif) ligand 4 antibody
      • CXCL 4 antibody
      • CXCL4 antibody
      • Iroplact antibody
      • MGC138298 antibody
      • Oncostatin A antibody
      • Oncostatin-A antibody
      • OncostatinA antibody
      • PF 4 antibody
      • PF-4 antibody
      • PF4 antibody
      • Platelet factor 4 antibody
      • PLF4_HUMAN antibody
      • SCYB 4 antibody
      • SCYB4 antibody
      • short form antibody
      • Small inducible cytokine subfamily member 4 antibody
      see all

    Images

    • Western blot - Anti-PF4 antibody (ab9561)
      Western blot - Anti-PF4 antibody (ab9561)
      Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 11 kDa
      Observed band size: 11 kDa
      Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes

    References

    This product has been referenced in:
    • Maybin JA  et al. Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair. J Clin Endocrinol Metab 102:1851-1860 (2017). Read more (PubMed: 28323919) »
    • Izumi M  et al. Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty. Arthritis Res Ther 18:191 (2016). Human . Read more (PubMed: 27558507) »

    See all 6 Publications for this product

    Publishing research using ab9561? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    I had a few question regarding platelet factor 4 antibody catalog number: ab9561. I need a neutralizing antibody that I can just add to my solution containing PF4. On you website it says that this antibody is neutralizing. I was wondering if this is te...

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    Thank you for contacting us.

    Ab9561 has been used in neutralizing assays and has been published byLambert MPet al.Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications.Blood110...

    Read More

    Western blot abreview for Anti-PF4 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Platelet lysate)
    Loading amount
    50 µg
    Specification
    Platelet lysate
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris Hcl gel)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Verified customer

    Submitted Aug 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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