Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17279-1] to PF4
- Suitable for: WB, IP
- Reacts with: Mouse
Product nameAnti-PF4 antibody [EPR17279-1]
See all PF4 primary antibodies
DescriptionRabbit monoclonal [EPR17279-1] to PF4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse PF4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Z126
- WB: Mouse serum and platelet lysate. IP: Mouse serum.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 11 kDa.|
FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsBinds non-covalently to a proteoglycan molecule.
- Information by UniProt
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution
Lane 1 : Mouse serum lysate
Lane 2 : Mouse platelet lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Blocking buffer: 5% NFDM/TBST.
Lane 1: 3 minutes;
Lane 2: 1 second.
PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) used at 1/10,000 dilution.
Lane 1: Mouse serum lysate 10 µg (Input).
Lane 2: ab182988 IP in mouse serum lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab182988 has not yet been referenced specifically in any publications.