Recombinant Anti-PF4 antibody [EPR17279-1] (ab182988)
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- References
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Overview
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Product name
Anti-PF4 antibody [EPR17279-1]
See all PF4 primary antibodies -
Description
Rabbit monoclonal [EPR17279-1] to PF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse PF4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Z126 -
Positive control
- WB: Mouse serum and platelet lysate. IP: Mouse serum.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17279-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab182988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 11 kDa. | |
| IP | 1/30. |
Target
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Function
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsBinds non-covalently to a proteoglycan molecule. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 56744 Mouse
- SwissProt: Q9Z126 Mouse
- Unigene: 332490 Mouse
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Alternative names
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
Images
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![Western blot - Anti-PF4 antibody [EPR17279-1] (ab182988)](https://www.abcam.com/ps/products/182/ab182988/Images/ab182988-289844-anti-pf4-antibody-epr17279-1-western-blot.jpg)
Western blot - Anti-PF4 antibody [EPR17279-1] (ab182988)All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution
Lane 1 : Mouse serum lysate
Lane 2 : Mouse platelet lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking buffer: 5% NFDM/TBST.
Exposure times:
Lane 1: 3 minutes;
Lane 2: 1 second.
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![Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] (ab182988)](https://www.abcam.com/ps/products/182/ab182988/Images/ab182988-289843-anti-pf4-antibody-epr17279-1-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PF4 antibody [EPR17279-1] (ab182988)PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) used at 1/10,000 dilution.
Lane 1: Mouse serum lysate 10 µg (Input).
Lane 2: ab182988 IP in mouse serum lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Datasheets and documents
References
ab182988 has not yet been referenced specifically in any publications.
