Product nameAnti-PF4 antibody [EPR17279-1]
See all PF4 primary antibodies
DescriptionRabbit monoclonal [EPR17279-1] to PF4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse PF4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Z126
- WB: Mouse serum and platelet lysate. IP: Mouse serum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 11 kDa.|
FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsBinds non-covalently to a proteoglycan molecule.
- Information by UniProt
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution
Lane 1 : Mouse serum lysate
Lane 2 : Mouse platelet lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Blocking buffer: 5% NFDM/TBST.
Lane 1: 3 minutes;
Lane 2: 1 second.
PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) used at 1/10,000 dilution.
Lane 1: Mouse serum lysate 10 µg (Input).
Lane 2: ab182988 IP in mouse serum lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab182988 has not yet been referenced specifically in any publications.