Recombinant
RabMAb

Recombinant Anti-PF4 antibody [EPR17279-1] - BSA and Azide free (ab227485)

Overview

  • Product name

    Anti-PF4 antibody [EPR17279-1] - BSA and Azide free
    See all PF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR17279-1] to PF4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse PF4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Z126

  • Positive control

    • WB: Mouse serum and platelet lysate.
  • General notes

    Ab227485 is the carrier-free version of ab182988. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227485 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
IP Use at an assay dependent concentration.

Target

  • Function

    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 4 antibody
    • Chemokine (C X C motif) ligand 4 antibody
    • Chemokine (CXC motif) ligand 4 antibody
    • chemokine ligand 4 antibody
    • CXCL 4 antibody
    • CXCL4 antibody
    • Iroplact antibody
    • MGC138298 antibody
    • Oncostatin A antibody
    • Oncostatin-A antibody
    • OncostatinA antibody
    • PF 4 antibody
    • PF-4 antibody
    • PF4 antibody
    • Pf4a antibody
    • Platelet factor 4 antibody
    • PLF4_HUMAN antibody
    • SCYB 4 antibody
    • SCYB4 antibody
    • short form antibody
    • Small inducible cytokine subfamily B antibody
    • Small inducible cytokine subfamily member 4 antibody
    see all

Images

  • PF4 was immunoprecipitated from 0.35 mg mouse serum lysate with ab182988 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab182988 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10,000 dilution.
    Lane 1: Mouse serum lysate 10 µg (Input).
    Lane 2: ab182988 IP in mouse serum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182988 in mouse serum (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182988).

  • All lanes : Anti-PF4 antibody [EPR17279-1] (ab182988) at 1/1000 dilution

    Lane 1 : Mouse serum lysate
    Lane 2 : Mouse platelet lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes;

    Lane 2: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182988).

References

ab227485 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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