Recombinant Anti-PF4 antibody [EPR17279-75] (ab222540)
![Sandwich ELISA - Anti-PF4 antibody [EPR17279-75] (ab222540)](https://www.abcam.com/ps/products/222/ab222540/Images/ab222540-281868-Mouse-PF4.jpg "Sandwich ELISA - Anti-PF4 antibody [EPR17279-75] (ab222540)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17279-75] to PF4
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
Overview
-
Product name
Anti-PF4 antibody [EPR17279-75]
See all PF4 primary antibodies -
Description
Rabbit monoclonal [EPR17279-75] to PF4 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17279-75 -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab222540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Target
-
Function
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsBinds non-covalently to a proteoglycan molecule. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 56744 Mouse
- SwissProt: Q9Z126 Mouse
- Unigene: 332490 Mouse
-
Alternative names
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
Images
-
Sandwich ELISA - Anti-PF4 antibody [EPR17279-75] (ab222540)
Example of Mouse PF4 standard curve in 1X Cell Extraction Buffer PTR using recombinant PF4 RabMAb antibody (ab222540) and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.
For a matched antibody pair set, see Mouse PF4 Matched Antibody Pair Kit (ab211763).
For a complete ELISA kit, see Mouse PF4 ELISA kit (ab202403).
-
![Anti-PF4 antibody [EPR17279-75] (ab222540)](https://www.abcam.com/ps/products/222/ab222540/Images/ab222540-2-benefits-of-recombinant-antibodies.png)
Anti-PF4 antibody [EPR17279-75] (ab222540)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab222540 has not yet been referenced specifically in any publications.
