Overview

  • Product name

    Anti-PF4 antibody [EPR7763]
    See all PF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7763] to PF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PF4 (P02776).

  • Positive control

    • Human spleen and platelet lysates, Human spleen tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Binds non-covalently to a proteoglycan molecule.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 4 antibody
    • Chemokine (C X C motif) ligand 4 antibody
    • Chemokine (CXC motif) ligand 4 antibody
    • CXCL 4 antibody
    • CXCL4 antibody
    • Iroplact antibody
    • MGC138298 antibody
    • Oncostatin A antibody
    • Oncostatin-A antibody
    • OncostatinA antibody
    • PF 4 antibody
    • PF-4 antibody
    • PF4 antibody
    • Platelet factor 4 antibody
    • PLF4_HUMAN antibody
    • SCYB 4 antibody
    • SCYB4 antibody
    • short form antibody
    • Small inducible cytokine subfamily member 4 antibody
    see all

Images

  • ab129183, at a 1/100 dilution, staining PF4 in paraffin embedded Human spleen tissue by Immunohistochemistry.
  • All lanes : Anti-PF4 antibody [EPR7763] (ab129183) at 1/1000 dilution

    Lane 1 : Human spleen lysates
    Lane 2 : Human platelet lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11 kDa

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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