Product nameAnti-PF4 antibody [EPR7763]
See all PF4 primary antibodies
DescriptionRabbit monoclonal [EPR7763] to PF4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to residues in Human PF4 (P02776).
- Human spleen and platelet lysates, Human spleen tissue
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (KD)KD = 5.40 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 11 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsBinds non-covalently to a proteoglycan molecule.
- Information by UniProt
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
ab129183, at a 1/100 dilution, staining PF4 in paraffin embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-PF4 antibody [EPR7763] (ab129183) at 1/1000 dilution
Lane 1 : Human spleen lysates
Lane 2 : Human platelet lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Chacón-Fernández P et al. Brain-derived Neurotrophic Factor in Megakaryocytes. J Biol Chem 291:9872-81 (2016). Read more (PubMed: 27006395) »
- Li J et al. CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction. J Immunol Res 2016:9276986 (2016). IHC . Read more (PubMed: 28053995) »