Overview

  • Product name

    Anti-PFDN1 antibody [EPR8547]
    See all PFDN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8547] to PFDN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human PFDN1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, HepG2, HT1080, PC12 and NIH 3T3 lysates; Human hepatocellular carcinoma tissue; Human kidney tissue; PC12 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    Images

    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PFDN1 with ab151708 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-PFDN1 antibody [EPR8547] (ab151708) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HT1080 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PFDN1 with ab151708 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow cytometric analysis of permeabilized PC12 cells labeling PFDN1 with ab151708 at 1/100 dilution (red). Rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Chen X  et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). Read more (PubMed: 28858244) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151708.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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