Product nameAnti-PFDN2 antibody - C-terminal
See all PFDN2 primary antibodies
DescriptionRabbit polyclonal to PFDN2 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab206691 was affinity purified using an epitope specific to PFDN2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab206691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 17 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Sequence similaritiesBelongs to the prefoldin subunit beta family.
Cellular localizationNucleus. Cytoplasm. Mitochondrion.
- Information by UniProt
- OTTHUMP00000029707 antibody
- PFD2 antibody
- PFD2_HUMAN antibody
All lanes : Anti-PFDN2 antibody - C-terminal (ab206691) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDa
Exposure time: 10 seconds
4-12% Bis-Tris gel
Detection of PFDN2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206691 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab206691 has not yet been referenced specifically in any publications.