Overview

  • Product name

    Anti-PFDN5 antibody [EPR7755]
    See all PFDN5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7755] to PFDN5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PFDN5 (Q99471).

  • Positive control

    • Human colon carcinoma tissue; 293T, HeLa, HepG2, fetal brain, fetal heart, fetal kidney, fetal muscle, Human spleen, NIH/3T3 and F9 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
    • Tissue specificity

      Highly expressed in pancreas and skeletal muscle and moderately in other tissues.
    • Sequence similarities

      Belongs to the prefoldin subunit alpha family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1190001O17Rik antibody
      • 1700010A06Rik antibody
      • c myc binding protein antibody
      • c-myc binding protein MM 1 antibody
      • C-myc-binding protein Mm-1 antibody
      • D15Ertd697e antibody
      • EIG 1 antibody
      • Eig1 antibody
      • MGC5329 antibody
      • MGC71907 antibody
      • MM 1 antibody
      • MM1 antibody
      • Myc modulator 1 antibody
      • PFD5 antibody
      • PFD5_HUMAN antibody
      • PFDN5 antibody
      • Prefoldin 5 antibody
      • Prefoldin subunit 5 antibody
      see all

    Images

    • All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Fetal heart lysate
      Lane 5 : Fetal kidney lysate
      Lane 6 : Human spleen lysate
      Lane 7 : HepG2 cell lysate
      Lane 8 : NIH/3T3 lysate
      Lane 9 : F9 cell lysate
      Lane 10 : Fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry.

    References

    This product has been referenced in:

    • Han A  et al. p63a modulates c-Myc activity via direct interaction and regulation of MM1 protein stability. Oncotarget 7:44277-44287 (2016). WB, IP ; Human . Read more (PubMed: 27341130) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129116.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up