Recombinant Anti-PFDN5 antibody [EPR7755] (ab129116)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-PFDN5 antibody [EPR7755]
See all PFDN5 primary antibodies -
Description
Rabbit monoclonal [EPR7755] to PFDN5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human PFDN5 (Q99471).
-
Positive control
- Human colon carcinoma tissue; 293T, HeLa, HepG2, fetal brain, fetal heart, fetal kidney, fetal muscle, Human spleen, NIH/3T3 and F9 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7755 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 17 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. -
Tissue specificity
Highly expressed in pancreas and skeletal muscle and moderately in other tissues. -
Sequence similarities
Belongs to the prefoldin subunit alpha family. - Information by UniProt
-
Database links
- Entrez Gene: 5204 Human
- Entrez Gene: 56612 Mouse
- Entrez Gene: 300257 Rat
- Omim: 604899 Human
- SwissProt: Q99471 Human
- SwissProt: Q9WU28 Mouse
- Unigene: 655327 Human
- Unigene: 158264 Mouse
see all -
Alternative names
- 1190001O17Rik antibody
- 1700010A06Rik antibody
- c myc binding protein antibody
see all
Images
-
![Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)](https://www.abcam.com/ps/products/129/ab129116/Images/ab129116-143128-anti-pfdn5-antibody-epr7755-western-blot.jpg)
Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain lysate
Lane 4 : Fetal heart lysate
Lane 5 : Fetal kidney lysate
Lane 6 : Human spleen lysate
Lane 7 : HepG2 cell lysate
Lane 8 : NIH/3T3 lysate
Lane 9 : F9 cell lysate
Lane 10 : Fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)](https://www.abcam.com/ps/products/129/ab129116/Images/ab129116-143127-anti-pfdn5-antibody-epr7755-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Han A et al. p63a modulates c-Myc activity via direct interaction and regulation of MM1 protein stability. Oncotarget 7:44277-44287 (2016). WB, IP ; Human . Read more (PubMed: 27341130) »
