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RecombinantRabMAb

Recombinant Anti-PFDN5 antibody [EPR7755] (ab129116)

  • Datasheet
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Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)
  • Anti-PFDN5 antibody [EPR7755] (ab129116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7755] to PFDN5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

You may also be interested in

Protein
Product image
Recombinant Human PFDN5 protein (ab113137)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-PFDN5 antibody [EPR7755]
    See all PFDN5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7755] to PFDN5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human PFDN5 (Q99471).

  • Positive control

    • Human colon carcinoma tissue; 293T, HeLa, HepG2, fetal brain, fetal heart, fetal kidney, fetal muscle, Human spleen, NIH/3T3 and F9 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7755
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human PFDN5 protein (ab113137)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
    • Tissue specificity

      Highly expressed in pancreas and skeletal muscle and moderately in other tissues.
    • Sequence similarities

      Belongs to the prefoldin subunit alpha family.
    • Target information above from: UniProt accession Q99471 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5204 Human
      • Entrez Gene: 56612 Mouse
      • Entrez Gene: 300257 Rat
      • Omim: 604899 Human
      • SwissProt: Q99471 Human
      • SwissProt: Q9WU28 Mouse
      • Unigene: 655327 Human
      • Unigene: 158264 Mouse
      • Unigene: 3401 Rat
      see all
    • Alternative names

      • 1190001O17Rik antibody
      • 1700010A06Rik antibody
      • c myc binding protein antibody
      • c-myc binding protein MM 1 antibody
      • C-myc-binding protein Mm-1 antibody
      • D15Ertd697e antibody
      • EIG 1 antibody
      • Eig1 antibody
      • MGC5329 antibody
      • MGC71907 antibody
      • MM 1 antibody
      • MM1 antibody
      • Myc modulator 1 antibody
      • PFD5 antibody
      • PFD5_HUMAN antibody
      • PFDN5 antibody
      • Prefoldin 5 antibody
      • Prefoldin subunit 5 antibody
      see all

    Images

    • Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)
      Western blot - Anti-PFDN5 antibody [EPR7755] (ab129116)
      All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Fetal heart lysate
      Lane 5 : Fetal kidney lysate
      Lane 6 : Human spleen lysate
      Lane 7 : HepG2 cell lysate
      Lane 8 : NIH/3T3 lysate
      Lane 9 : F9 cell lysate
      Lane 10 : Fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] (ab129116)

      ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-PFDN5 antibody [EPR7755] (ab129116)
      Anti-PFDN5 antibody [EPR7755] (ab129116)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab129116? Please let us know so that we can cite the reference in this datasheet.

    ab129116 has been referenced in 2 publications.

    • Chen Y  et al. ?Np63a down-regulates c-Myc modulator MM1 via E3 ligase HERC3 in the regulation of cell senescence. Cell Death Differ 25:2118-2129 (2018). PubMed: 29880857
    • Han A  et al. p63a modulates c-Myc activity via direct interaction and regulation of MM1 protein stability. Oncotarget 7:44277-44287 (2016). WB, IP ; Human . PubMed: 27341130

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab129116.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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