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Rabbit polyclonal to PFKFB1
HumanPredicted to work with:
Rat, Cow, Pig
Recombinant fragment, corresponding to a region within the amino acids 1-194 of Human PFKFB1 (Uniprot ID: P16118).
- H1299 & HeLa whole cell lysate, HeLa cells, H358 xenograft, 293T, MOLT4, Raji.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 55 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
||1/100 - 1/1000.
Synthesis and degradation of fructose 2,6-bisphosphate.
In the C-terminal section; belongs to the phosphoglycerate mutase family.
Information by UniProt
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 1 antibody
- 6-bisphosphatase antibody
- 6-P2ase 1 antibody
Western blot - Anti-PFKFB1 antibody (ab154573)
All lanes : Anti-PFKFB1 antibody (ab154573) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (ab154573)
Immunofluorescence analysis of methanol-fixed HeLa cells labeling PFKFB1 with ab154573 at 1/200 dilution (Green). Lower panel co-stained with Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (ab154573)
Immunohistochemical analysis of paraffin-embedded H358 xenograft labeling PFKFB1 with ab154573 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"