Key features and details
- Rabbit polyclonal to PFKFB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PFKFB1 antibody
See all PFKFB1 primary antibodies
DescriptionRabbit polyclonal to PFKFB1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Pig
Recombinant fragment, corresponding to a region within the amino acids 1-194 of Human PFKFB1 (Uniprot ID: P16118).
- H1299 & HeLa whole cell lysate, HeLa cells, H358 xenograft, 293T, MOLT4, Raji.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
|ICC/IF||1/100 - 1/1000.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 1 antibody
- 6-bisphosphatase antibody
- 6-P2ase 1 antibody
All lanes : Anti-PFKFB1 antibody (ab154573) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells labeling PFKFB1 with ab154573 at 1/200 dilution (Green). Lower panel co-stained with Hoechst 33342 (blue).
Immunohistochemical analysis of paraffin-embedded H358 xenograft labeling PFKFB1 with ab154573 at 1/500 dilution.
ab154573 has not yet been referenced specifically in any publications.