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Signal Transduction Metabolism Energy Metabolism
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Anti-PFKFB1 antibody (ab155564)

  • Datasheet
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Western blot - Anti-PFKFB1 antibody (ab155564)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (ab155564)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (ab155564)

Key features and details

  • Rabbit polyclonal to PFKFB1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PFKFB1 antibody
    See all PFKFB1 primary antibodies
  • Description

    Rabbit polyclonal to PFKFB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Mouse PFKFB1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P70266

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Liver tissue lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2 This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal liver

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PFKFB1 protein (ab151800)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
WB
Mouse
Rat
Human
All applications
Sheep
Rabbit
Horse
Cow
Chimpanzee
Orangutan
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Synthesis and degradation of fructose 2,6-bisphosphate.
  • Tissue specificity

    Liver.
  • Sequence similarities

    In the C-terminal section; belongs to the phosphoglycerate mutase family.
  • Target information above from: UniProt accession P16118 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5207 Human
    • Entrez Gene: 5208 Human
    • Entrez Gene: 5209 Human
    • Entrez Gene: 5210 Human
    • Entrez Gene: 18639 Mouse
    • Entrez Gene: 18640 Mouse
    • Entrez Gene: 270198 Mouse
    • Entrez Gene: 117276 Rat
    • Entrez Gene: 24638 Rat
    • Entrez Gene: 24640 Rat
    • Entrez Gene: 54283 Rat
    • Omim: 311790 Human
    • SwissProt: O60825 Human
    • SwissProt: P16118 Human
    • SwissProt: Q16875 Human
    • SwissProt: Q16877 Human
    • SwissProt: P70265 Mouse
    • SwissProt: P70266 Mouse
    • SwissProt: Q6DTY7 Mouse
    • SwissProt: O35552 Rat
    • SwissProt: P07953 Rat
    • SwissProt: P25114 Rat
    • SwissProt: Q9JJH5 Rat
    • Unigene: 195471 Human
    • Unigene: 282702 Human
    • Unigene: 444304 Human
    • Unigene: 476217 Human
    • Unigene: 132391 Mouse
    • Unigene: 249131 Mouse
    • Unigene: 249861 Mouse
    • Unigene: 10115 Rat
    • Unigene: 10791 Rat
    • Unigene: 44844 Rat
    • Unigene: 90092 Rat
    see all
  • Alternative names

    • 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 1 antibody
    • 6-bisphosphatase antibody
    • 6-P2ase 1 antibody
    • 6-P2ASE liver isozyme antibody
    • 6PF 2 K/Fru 2 6 P2ASE liver isozyme antibody
    • 6PF 2 K/Fru 2,6 P2ase 1 antibody
    • 6PF-2-K/Fru-2 antibody
    • F261_HUMAN antibody
    • F6PK antibody
    • Fructose 2 6 bisphosphatase antibody
    • Fructose 6 phosphate 2 kinase:fructose 2 6 bisphosphatase antibody
    • Fructose-2 antibody
    • HL2K antibody
    • MGC116715 antibody
    • MGC116717 antibody
    • PFK/FBPase 1 antibody
    • PFKFB 1 antibody
    • Pfkfb1 antibody
    • PFRX antibody
    see all

Images

  • Western blot - Anti-PFKFB1 antibody (ab155564)
    Western blot - Anti-PFKFB1 antibody (ab155564)
    All lanes : Anti-PFKFB1 antibody (ab155564) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 16 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155564 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (ab155564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB1 antibody (ab155564)

    IHC image of TPFKFB1 staining in Mouse normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab155564, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (ab155564)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKFB1 antibody (ab155564)

    ICC/IF image of ab155564 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155564 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab155564? Please let us know so that we can cite the reference in this datasheet.

    ab155564 has not yet been referenced specifically in any publications.

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