Anti-PFKFB2 antibody (ab234865)
Key features and details
- Rabbit polyclonal to PFKFB2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PFKFB2 antibody
See all PFKFB2 primary antibodies -
Description
Rabbit polyclonal to PFKFB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human PFKFB2 aa 350 to the C-terminus.
Database link: O60825 -
Positive control
- WB: K562 whole cell lysate; Rat brain and kidney lysates. IHC-P: Human lung cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/5000. Predicted molecular weight: 58 kDa.
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/200 - 1/5000. Predicted molecular weight: 58 kDa. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Synthesis and degradation of fructose 2,6-bisphosphate. -
Tissue specificity
Heart. -
Sequence similarities
In the C-terminal section; belongs to the phosphoglycerate mutase family. - Information by UniProt
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Database links
- Entrez Gene: 287019 Cow
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Entrez Gene: 18639 Mouse
- Entrez Gene: 18640 Mouse
- Entrez Gene: 270198 Mouse
see all -
Alternative names
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody
- 6-bisphosphatase antibody
- 6-P2ase 2 antibody
see all
Images
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All lanes : Anti-PFKFB2 antibody (ab234865) at 1/200 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa -
Paraffin-embedded human lung cancer tissue stained for PFKFB2 using ab234865 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PFKFB2 (green) using ab234865 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234865 has been referenced in 1 publication.
- Pan X et al. miR-1297 Suppresses Osteosarcoma Proliferation and Aerobic Glycolysis by Regulating PFKFB2. Onco Targets Ther 13:11265-11275 (2020). PubMed: 33173315