Overview

  • Product name

  • Description

    Rabbit polyclonal to PFKFB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PFKFB2 aa 455-505. The exact sequence is proprietary. (NP_006203.2).

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-239T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.

Target

Images

  • All lanes : Anti-PFKFB2 antibody (ab241506) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 58 kDa


    Exposure time: 30 seconds
  • PFKFB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
    ab241506 used for IP at 6 µg per reaction. Western blot was perfomed on the immunoprecipitate using ab241506 at 0.4 µg/ml.

    Lane 1: ab241506 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab241506 has not yet been referenced specifically in any publications.

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