Key features and details
- Rabbit polyclonal to PFKFB2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PFKFB2 antibody
See all PFKFB2 primary antibodies
DescriptionRabbit polyclonal to PFKFB2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human PFKFB2 aa 455-505. The exact sequence is proprietary. (NP_006203.2).
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-239T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241506 was affinity purified using an epitope specific to PFKFB2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 58 kDa.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody
- 6-bisphosphatase antibody
- 6-P2ase 2 antibody
All lanes : Anti-PFKFB2 antibody (ab241506) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 seconds
PFKFB2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab241506 used for IP at 6 µg per reaction. Western blot was perfomed on the immunoprecipitate using ab241506 at 0.4 µg/ml.
Lane 1: ab241506 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241506 has not yet been referenced specifically in any publications.