Product nameAnti-PFKFB2 antibody
See all PFKFB2 primary antibodies
DescriptionRabbit polyclonal to PFKFB2
SpecificityThis antibody detects endogenous levels of total PFKFB2 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
A synthesized peptide derived from the N-terminal of human PFKFB2.
- Extracts from Jurkat cells This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab70175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody
- 6-bisphosphatase antibody
- 6-P2ase 2 antibody
ICC/IF image of ab70175 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70175 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-PFKFB2 antibody (ab70175) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Various other bands are detected, from 26 to over 117kDa.
Anti-PFKFB2 antibody (ab70175) at 1/500 dilution (in 5% Marvel TBS-T for 24 hours at 22°C) + Whole cell lysate of mouse NIH 3T£ cells at 25 µg
An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution
Predicted band size: 58 kDa
Exposure time: 8 minutes
Blocking Step: 5% BSA for 1 hour at 22°C
ab70175 has not yet been referenced specifically in any publications.