Anti-PFKFB2 antibody (ab70175)
Key features and details
- Rabbit polyclonal to PFKFB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFKFB2 antibody
See all PFKFB2 primary antibodies -
Description
Rabbit polyclonal to PFKFB2 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total PFKFB2 protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from the N-terminal of human PFKFB2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 58 kDa. |
Target
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Function
Synthesis and degradation of fructose 2,6-bisphosphate. -
Tissue specificity
Heart. -
Sequence similarities
In the C-terminal section; belongs to the phosphoglycerate mutase family. - Information by UniProt
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Database links
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Omim: 171835 Human
- SwissProt: O60825 Human
- SwissProt: P16118 Human
- SwissProt: Q16875 Human
see all -
Alternative names
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody
- 6-bisphosphatase antibody
- 6-P2ase 2 antibody
see all
Images
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Western blot - Anti-PFKFB2 antibody (ab70175)All lanes : Anti-PFKFB2 antibody (ab70175) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Various other bands are detected, from 26 to over 117kDa. -
Western blot - Anti-PFKFB2 antibody (ab70175)This image is courtesy of an anonymous AbreviewAnti-PFKFB2 antibody (ab70175) at 1/500 dilution (in 5% Marvel TBS-T for 24 hours at 22°C) + Whole cell lysate of mouse NIH 3T£ cells at 25 µg
Secondary
An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution
Predicted band size: 58 kDa
Exposure time: 8 minutes
Blocking Step: 5% BSA for 1 hour at 22°C
Datasheets and documents
References (1)
ab70175 has been referenced in 1 publication.
- Liu H et al. miR-613 inhibits Warburg effect in gastric cancer by targeting PFKFB2. Biochem Biophys Res Commun 515:37-43 (2019). PubMed: 31122697
