Key features and details
- Rabbit polyclonal to PFKFB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PFKFB2 antibody
See all PFKFB2 primary antibodies
DescriptionRabbit polyclonal to PFKFB2
SpecificityThis antibody detects endogenous levels of total PFKFB2 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
A synthesized peptide derived from the N-terminal of human PFKFB2.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab70175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2 antibody
- 6-bisphosphatase antibody
- 6-P2ase 2 antibody
All lanes : Anti-PFKFB2 antibody (ab70175) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Various other bands are detected, from 26 to over 117kDa.
Anti-PFKFB2 antibody (ab70175) at 1/500 dilution (in 5% Marvel TBS-T for 24 hours at 22°C) + Whole cell lysate of mouse NIH 3T£ cells at 25 µg
An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution
Predicted band size: 58 kDa
Exposure time: 8 minutes
Blocking Step: 5% BSA for 1 hour at 22°C
ab70175 has been referenced in 1 publication.
- Liu H et al. miR-613 inhibits Warburg effect in gastric cancer by targeting PFKFB2. Biochem Biophys Res Commun 515:37-43 (2019). PubMed: 31122697