Overview

  • Product name

  • Description

    Rabbit polyclonal to PFKFB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human PFKFB3 aa 425-475. The exact sequence is proprietary. NP_004557.1
    Database link: Q16875

  • Positive control

    • IP: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Synthesis and degradation of fructose 2,6-bisphosphate.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      In the C-terminal section; belongs to the phosphoglycerate mutase family.
    • Post-translational
      modifications

      Phosphorylation by AMPK stimulates activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
      • 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
      • 6-bisphosphatase antibody
      • 6-P2ase 3 antibody
      • 6-P2ASE brain/placenta-type isozyme antibody
      • 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody
      • 6PF 2-K/Fru 2,6 P2ase 3 antibody
      • 6PF-2-K/Fru-2 antibody
      • F263_HUMAN antibody
      • fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase antibody
      • Fructose-2 antibody
      • Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody
      • iPFK 2 antibody
      • iPFK-2 antibody
      • IPFK2 antibody
      • PFK/FBPase 3 antibody
      • PFK2 antibody
      • PFKFB3 antibody
      • Renal carcinoma antigen NY REN 56 antibody
      • Renal carcinoma antigen NY-REN-56 antibody
      • uPFK antibody
      see all

    Images

    • PFKFB3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226262 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different rabbit anti-PFKFB3 antibody at 1 µg/ml.

      Lane 1: ab226262 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab226262 has not yet been referenced specifically in any publications.

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