Product nameAnti-PFKFB3 antibody
See all PFKFB3 primary antibodies
DescriptionRabbit polyclonal to PFKFB3
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human PFKFB3 aa 425-475. The exact sequence is proprietary. NP_004557.1
Database link: Q16875
- IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226262 was affinity purified using an epitope specific to PFKFB3 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
modificationsPhosphorylation by AMPK stimulates activity.
- Information by UniProt
- 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
- 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
- 6-bisphosphatase antibody
PFKFB3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226262 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different rabbit anti-PFKFB3 antibody at 1 µg/ml.
Lane 1: ab226262 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226262 has not yet been referenced specifically in any publications.