Key features and details
- Rabbit polyclonal to PFKFB3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PFKFB3 antibody
See all PFKFB3 primary antibodies
DescriptionRabbit polyclonal to PFKFB3
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human PFKFB3 aa 425-475. The exact sequence is proprietary. NP_004557.1
Database link: Q16875
- IP: Jurkat whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226262 was affinity purified using an epitope specific to PFKFB3 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
modificationsPhosphorylation by AMPK stimulates activity.
- Information by UniProt
- 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
- 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
- 6-bisphosphatase antibody
PFKFB3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226262 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different rabbit anti-PFKFB3 antibody at 1 µg/ml.
Lane 1: ab226262 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226262 has not yet been referenced specifically in any publications.