Recombinant
RabMAb

Recombinant Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291)

Overview

  • Product name

    Anti-PFKFB3 (phospho S461) antibody [EPR19735]
    See all PFKFB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR19735] to PFKFB3 (phospho S461)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human PFKFB3 aa 450 to the C-terminus (phospho S461). The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293T transfected with mPFKFB3 (WT) whole cell lysate. ICC/IF: Flag-mPFKFB3 transfected HEK-293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF 1/100.

Target

  • Function

    Synthesis and degradation of fructose 2,6-bisphosphate.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    In the C-terminal section; belongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications

    Phosphorylation by AMPK stimulates activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
    • 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
    • 6-bisphosphatase antibody
    • 6-P2ase 3 antibody
    • 6-P2ASE brain/placenta-type isozyme antibody
    • 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody
    • 6PF 2-K/Fru 2,6 P2ase 3 antibody
    • 6PF-2-K/Fru-2 antibody
    • F263_HUMAN antibody
    • fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase antibody
    • Fructose-2 antibody
    • Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody
    • iPFK 2 antibody
    • iPFK-2 antibody
    • IPFK2 antibody
    • PFK/FBPase 3 antibody
    • PFK2 antibody
    • PFKFB3 antibody
    • Renal carcinoma antigen NY REN 56 antibody
    • Renal carcinoma antigen NY-REN-56 antibody
    • uPFK antibody
    see all

Images

  • All lanes : Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (vector control), whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT) whole cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 S461A mutant, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 2% BSA/TBST.

    The plasmids were kindly provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.

  • Dot blot analysis of PFKFB3 (phospho S461) labeled with ab202291 at 1/1000 dilution.

    Lane 1: PFKFB3 (phospho S461) peptide;
    Lane 2: PFKFB3 non-phospho peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 was used as secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling PFKFB3 (phospho S461) with ab202291 at 1/100 dilution. Counterstained with Anti-FLAG antibody (green).

    The results showed positive staining on Flag-mPFKFB3 transfected HEK-293T cells and no staining on Flag-mPFKFB3 S461A mutant transfected HEK-293T cells. The image was provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.

    The nuclear counter stain is DAPI (blue).

     

References

ab202291 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202291.
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