Product nameAnti-PFKFB3 (phospho S461) antibody [EPR19735]
See all PFKFB3 primary antibodies
DescriptionRabbit monoclonal [EPR19735] to PFKFB3 (phospho S461)
Tested applicationsSuitable for: Dot blot, WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide within Human PFKFB3 aa 450 to the C-terminus (phospho S461). The exact sequence is proprietary.
- WB: HEK-293T transfected with mPFKFB3 (WT) whole cell lysate. ICC/IF: Flag-mPFKFB3 transfected HEK-293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab202291 in the following tested applications.
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
modificationsPhosphorylation by AMPK stimulates activity.
- Information by UniProt
- 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
- 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
- 6-bisphosphatase antibody
All lanes : Anti-PFKFB3 (phospho S461) antibody [EPR19735] (ab202291) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with mPFKFB3 S461A mutant, whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes
Blocking/Dilution buffer: 2% BSA/TBST.
The plasmids were kindly provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.
Dot blot analysis of PFKFB3 (phospho S461) labeled with ab202291 at 1/1000 dilution.
Lane 1: PFKFB3 (phospho S461) peptide;
Lane 2: PFKFB3 non-phospho peptide.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling PFKFB3 (phospho S461) with ab202291 at 1/100 dilution. Counterstained with Anti-FLAG antibody (green).
The results showed positive staining on Flag-mPFKFB3 transfected HEK-293T cells and no staining on Flag-mPFKFB3 S461A mutant transfected HEK-293T cells. The image was provided by our collaborator Dr. Jianghuai Liu, Nanjing University MARC.
The nuclear counter stain is DAPI (blue).
ab202291 has not yet been referenced specifically in any publications.