Overview

  • Product name

  • Description

    Rabbit polyclonal to PFKFB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human PFKFB4 aa 200-469 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: HepG2 and Raji cell lysates; IHC-P: NCI-N87 xenograft, mouse spleen and testis tissue; ICC/IF: NT2D1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with a citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence of NT2D1 stainging PFKFB4 using ab137785 at a dilution of 1/500. Nuclear staining using Hoechst 33342.

  • Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution + Rat tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 54 kDa

  • Anti-PFKFB4 antibody (ab137785) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue labelling PFKFB4 with ab137785 at 1/500 dilution. Antigen retrieval performed with an EDTA based buffer, pH 8.0, 15 min

References

This product has been referenced in:

  • Ellis R  et al. Increased expression and phosphorylation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoforms in urinary exosomes in pre-eclampsia. J Transl Med 17:60 (2019). Read more (PubMed: 30819197) »
  • Gao R  et al. PFKFB4 Promotes Breast Cancer Metastasis via Induction of Hyaluronan Production in a p38-Dependent Manner. Cell Physiol Biochem 50:2108-2123 (2018). Read more (PubMed: 30415245) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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