Product nameAnti-PFKFB4 antibody
See all PFKFB4 primary antibodies
DescriptionRabbit polyclonal to PFKFB4
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human PFKFB4 aa 266-296 (internal sequence) conjugated to keyhole limpet haemocyanin.
- IHC-P:Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and mouse brain tissue lysate. WB: Raji, Molt-4, NCI-H1299, 293, and U-87 MG whole cell lysate ICC/IF: HepG2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab71622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 60 kDa and many other bands (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
- 6-bisphosphatase antibody
- 6-P2ase 4 antibody
- 6-P2ASE testis-type isozyme antibody
All lanes : Anti-PFKFB4 antibody (ab71622) at 1/1000 dilution
Lane 1 : Raji, whole cell lysate with 5% NFDM/TBST.
Lane 2 : Molt-4 whole cell lysate with 5% NFDM/TBST.
Lane 3 : NCI-H1299 whole cell lysate with 5% NFDM/TBST.
Lane 4 : 293 whole cell lysate Lane with 5% NFDM/TBST.
Lane 5 : U-87 MG whole cell lysate with 5% NFDM/TBST.
Lysates/proteins at 20 µg per lane.
All lanes : Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 54 kDa
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by AEC staining.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
ICC/IF image of ab71622 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab71622 has not yet been referenced specifically in any publications.