Anti-PFKL antibody (ab241077)

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Overview

  • Product name

    Anti-PFKL antibody
    See all PFKL primary antibodies

  • Description

    Rabbit polyclonal to PFKL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Synthetic peptide within Human PFKL aa 730-780. The exact sequence is proprietary. (NP_002617.3).

  • Positive control

    • IP: PFKL IP in HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • PFK liver isoenzyme antibody
        • 6 phosphofructokinase, liver type antibody
        • K6PL antibody
        • liver phosphofructokinase antibody
        • liver type 1 phosphofructokinase antibody
        • PFK B antibody
        • PFK liver isozyme antibody
        • Phosphofructo 1 kinase isozyme B antibody
        • Phosphofructokinase 1 antibody
        • Phosphofructokinase, liver antibody
        • Phosphohexokinase antibody
        see all

      Images

      • Immunoprecipitation - Anti-PFKL antibody (ab241077)
        Immunoprecipitation - Anti-PFKL antibody (ab241077)

        PFKL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241077 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241077 at 1 µg/ml.

        Lane 1: ab241077 IP in HeLa whole cell lysate.

        Lane 2: Control IgG IP in HeLa whole cell lysate.

        Detection: Chemiluminescence with exposure time of 3 minutes.

      References

      ab241077 has not yet been referenced specifically in any publications.

      Publishing research using ab241077? Please let us know so that we can cite the reference in this datasheet.

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