Product nameAnti-PFKL antibody
See all PFKL primary antibodies
DescriptionRabbit polyclonal to PFKL
SpecificityThis antibody is specific for Fructose 6 Phosphate Kinase Liver type (isoenzyme B).
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
A KLH conjugated synthetic peptide selected within aa 650~700 of human PFKL (C-Terminal).
- HepG2 whole cell lysate (ab7900) and hepatocarcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab37583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/50 - 1/100.|
RelevancePFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.
- PFK liver isoenzyme antibody
- 6 phosphofructokinase, liver type antibody
- K6PL antibody
Anti-PFKL antibody (ab37583) at 1/100 dilution + HepG2 cell lysate
Predicted band size: 85 kDa
Observed band size: 85 kDa
Staining of Fructose 6 Phosphate Kinase on Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue, using ab37583 at a 1/50 dilution (a peroxidase-conjugated the secondary antibody, followed by AEC staining was used).
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This product has been referenced in:
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). Read more (PubMed: 28607489) »
- Li B et al. Fructose-1,6-bisphosphatase opposes renal carcinoma progression. Nature 513:251-5 (2014). Read more (PubMed: 25043030) »