Anti-PFKL antibody (ab97443)
Key features and details
- Rabbit polyclonal to PFKL
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFKL antibody
See all PFKL primary antibodies -
Description
Rabbit polyclonal to PFKL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 475-691 of Human PFKL (NP_001002021)
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Positive control
- A431, A549, H1299, HeLa and HepG2 cell lines.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 90 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Relevance
PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 508683 Cow
- Entrez Gene: 5211 Human
- Entrez Gene: 18641 Mouse
- Entrez Gene: 25741 Rat
- Omim: 171860 Human
- SwissProt: A1A4J1 Cow
- SwissProt: P17858 Human
- SwissProt: P12382 Mouse
see all -
Alternative names
- PFK liver isoenzyme antibody
- 6 phosphofructokinase, liver type antibody
- K6PL antibody
see all
Images
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Western blot - Anti-PFKL antibody (ab97443)Anti-PFKL antibody (ab97443) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody (ab97443)Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97443 at 1/200 dilution (upper panel) and merged with DNA probe (lower panel).
Protocols
References (0)
ab97443 has not yet been referenced specifically in any publications.
