Anti-PFKL antibody (ab97443)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PFKL antibody
See all PFKL primary antibodies -
Description
Rabbit polyclonal to PFKL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 475-691 of Human PFKL (NP_001002021)
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Positive control
- A431, A549, H1299, HeLa and HepG2 cell lines.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 90 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Relevance
PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 508683 Cow
- Entrez Gene: 5211 Human
- Entrez Gene: 18641 Mouse
- Entrez Gene: 25741 Rat
- Omim: 171860 Human
- SwissProt: A1A4J1 Cow
- SwissProt: P17858 Human
- SwissProt: P12382 Mouse
see all -
Alternative names
- PFK liver isoenzyme antibody
- 6 phosphofructokinase, liver type antibody
- K6PL antibody
see all
Images
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Western blot - Anti-PFKL antibody (ab97443)Anti-PFKL antibody (ab97443) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody (ab97443)Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97443 at 1/200 dilution (upper panel) and merged with DNA probe (lower panel).
References
ab97443 has not yet been referenced specifically in any publications.
