Overview

  • Product name
    Anti-PFKL antibody
    See all PFKL primary antibodies
  • Description
    Rabbit polyclonal to PFKL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 475-691 of Human PFKL (NP_001002021)

  • Positive control
    • A431, A549, H1299, HeLa and HepG2 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab97443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 90 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance
    PFKL is the Liver isozyme of PFK (Phosphofructokinase), which is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits.
  • Cellular localization
    Cytoplasmic
  • Database links
    see all
  • Alternative names
    • PFK liver isoenzyme antibody
    • 6 phosphofructokinase, liver type antibody
    • K6PL antibody
    • liver phosphofructokinase antibody
    • liver type 1 phosphofructokinase antibody
    • PFK B antibody
    • PFK liver isozyme antibody
    • Phosphofructo 1 kinase isozyme B antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase, liver antibody
    • Phosphohexokinase antibody
    see all

Images

  • Western blot - Anti-PFKL antibody (ab97443)
    Western blot - Anti-PFKL antibody (ab97443)
    Anti-PFKL antibody (ab97443) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size: 90 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody (ab97443)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody (ab97443)
    Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97443 at 1/200 dilution (upper panel) and merged with DNA probe (lower panel).

References

ab97443 has not yet been referenced specifically in any publications.

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