Anti-PFKM antibody (ab237545)
Key features and details
- Rabbit polyclonal to PFKM
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PFKM antibody
See all PFKM primary antibodies -
Description
Rabbit polyclonal to PFKM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human PFKM aa 166-334.
Database link: P08237 -
Positive control
- WB: HeLa and HEK-293 whole cell lysate; Mouse heart tissue lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 85 kDa.
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 85 kDa. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
Involvement in disease
Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated. -
Sequence similarities
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5213 Human
- Entrez Gene: 18642 Mouse
- Omim: 610681 Human
- SwissProt: P08237 Human
- SwissProt: P47857 Mouse
- Unigene: 75160 Human
- Unigene: 272582 Mouse
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Alternative names
- 6 Phosphofructokinase Muscle Type antibody
- 6-phosphofructokinase antibody
- 6-phosphofructokinase muscle type antibody
see all
Images
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All lanes : Anti-PFKM antibody (ab237545) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Mouse heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa -
Paraffin-embedded human endometrial cancer tissue stained for PFKM using ab237545 at 1/100 dilution in immunohistochemical analysis.
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A549 (human lung carcinoma cell line) cells stained for PFKM (green) using ab237545 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237545 has not yet been referenced specifically in any publications.