Overview

  • Product name

  • Description

    Rabbit polyclonal to PFKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PFKM aa 166-334.
    Database link: P08237

  • Positive control

    • WB: HeLa and HEK-293 whole cell lysate; Mouse heart tissue lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: A549 cells.
  • General notes

     This product was previously labelled as Fructose 6 Phosphate Kinase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab237545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 85 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Involvement in disease

    Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Images

  • All lanes : Anti-PFKM antibody (ab237545) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Mouse heart tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 85 kDa

  • Paraffin-embedded human endometrial cancer tissue stained for PFKM using ab237545 at 1/100 dilution in immunohistochemical analysis.

  • A549 (human lung carcinoma cell line) cells stained for PFKM (green) using ab237545 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

References

ab237545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab237545.
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