Key features and details
- Rabbit polyclonal to PFKM
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PFKM antibody
See all PFKM primary antibodies
DescriptionRabbit polyclonal to PFKM
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human PFKM aa 166-334.
Database link: P08237
- WB: HeLa and HEK-293 whole cell lysate; Mouse heart tissue lysate. IHC-P: Human endometrial cancer tissue. ICC/IF: A549 cells.
This product was previously labelled as Fructose 6 Phosphate Kinase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 85 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Involvement in diseaseDefects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
- Information by UniProt
- 6 Phosphofructokinase Muscle Type antibody
- 6-phosphofructokinase antibody
- 6-phosphofructokinase muscle type antibody
All lanes : Anti-PFKM antibody (ab237545) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Mouse heart tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Paraffin-embedded human endometrial cancer tissue stained for PFKM using ab237545 at 1/100 dilution in immunohistochemical analysis.
A549 (human lung carcinoma cell line) cells stained for PFKM (green) using ab237545 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237545 has not yet been referenced specifically in any publications.