Overview

  • Product name

  • Description

    Rabbit polyclonal to PFKM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment corresponding to Human PFKM aa 1-223.

  • Positive control

    • 293T, A431, H1299, HeLa, Molt-4, Raji cells
  • General notes

     This product was previously labelled as Fructose 6 Phosphate Kinase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab97353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 85 kDa.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Involvement in disease

    Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Images

  • Anti-PFKM antibody (ab97353) at 1/5000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 85 kDa



    7.5% SDS PAGE

References

ab97353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
50 µg
Treatment
Different treatments
Specification
Muscle
Blocking step
2.5%BSA + 2.5% Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 30 2012

Question
Answer

Thank you for your reply.


Since this antibody has not been tested in mouse it is not guaranteed by the Abpromise. Instead, I have offered a testing discount off of a future purchase. Please let me know if you would be interested in taking advantage of this offer.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you very much for your interest in ab97353.

To our knowledge, ab97353 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab97353 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab97353 in mouse. I will then send a discount code. This code must be issued before purchasing ab97353 so please wait for my reply before ordering.

2. Purchase ab97353 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab97353 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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