RecombinantRabMAb

Recombinant Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

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Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
  • Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10734(B)] to PFKM - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free
    See all PFKM primary antibodies

  • Description

    Rabbit monoclonal [EPR10734(B)] to PFKM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PFKM. The exact sequence is proprietary.
    Database link: P08237
    (Peptide available as ab207505)

  • Positive control

    • ICC/IF: SH-SY5Y cells.

  • General notes

    ab232495 is the carrier-free version of ab154804 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Fructose 6 Phosphate Kinase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab232495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

  • Involvement in disease

    Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.

  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Images

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab232495? Please let us know so that we can cite the reference in this datasheet.

ab232495 has not yet been referenced specifically in any publications.

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