Recombinant
RabMAb

Recombinant Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

Submit a review Submit a question

Overview

  • Product name

    Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free
    See all PFKM primary antibodies

  • Description

    Rabbit monoclonal [EPR10734(B)] to PFKM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PFKM. The exact sequence is proprietary.
    Database link: P08237
    (Peptide available as ab207505)

  • Positive control

    • ICC/IF: SH-SY5Y cells.

  • General notes

    ab232495 is the carrier-free version of ab154804 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232495 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Fructose 6 Phosphate Kinase

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

  • Involvement in disease

    Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.

  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 6 Phosphofructokinase Muscle Type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase muscle type antibody
    • EC 2.7.1.1 antibody
    • EC 2.7.1.11 antibody
    • GSD7 antibody
    • K6PF_HUMAN antibody
    • MGC8699 antibody
    • muscle type antibody
    • PFK, muscle type antibody
    • PFK-A antibody
    • PFKA antibody
    • PFKL antibody
    • PFKM antibody
    • PFKP antibody
    • PFKX antibody
    • Phosphofructo 1 Kinase Isozyme A antibody
    • Phosphofructo-1-kinase isozyme A antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase M antibody
    • Phosphofructokinase, muscle antibody
    • Phosphofructokinase, muscle type antibody
    • Phosphofructokinase, polypeptide X antibody
    • Phosphofructokinase-M antibody
    • Phosphohexokinase antibody
    see all

Images

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Flow Cytometry - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKM antibody [EPR10734(B)] - BSA and Azide free (ab232495)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154804).

References

ab232495 has not yet been referenced specifically in any publications.

Publishing research using ab232495? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab232495.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com